| 测定狂犬病病毒中和抗体的微量免疫酶试验 |
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| 引用本文： | 夏平安,侯世宽,杨盛华,殷震. 测定狂犬病病毒中和抗体的微量免疫酶试验[J]. 中国兽医学报, 1991, 0(4) |
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| 作者姓名： | 夏平安 侯世宽 杨盛华 殷震 |
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| 作者单位： | 兽医大学军事兽医研究所(夏平安，侯世宽，杨盛华)，兽医大学军事兽医研究所(殷震) |
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| 摘 要： | 在Kazuakz等报道的微量免疫酶技术(MIET)的基础上,以健康细胞对照孔OD值均数加3倍标准差作为间接ELISA判定标准,进而建立了求中和剂量的新方法.通过用中和99%病毒的一致判定标准同经典的小鼠中和试验(MNT)比较看,本试验所建立的方法可代替MNT.
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| 关 键 词： | 狂犬病病毒 Flury—LEP株 中和抗体 微量免疫酶试验 |
Establishment of a Micro-immunoenzymatic Test for Detecting the Rabies Neutralizing Antibody |
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| Xia Pingan et al. Establishment of a Micro-immunoenzymatic Test for Detecting the Rabies Neutralizing Antibody[J]. Chinese Journal of Veterinary Science, 1991, 0(4) |
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| Authors: | Xia Pingan et al |
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| Abstract: | On the basis of the micro-immunoenzymatic technique developed by Kazuaki et al, a micro-immunoenzymatic test (MIET) for detecting the rabies neutralizing antibody has been established. The sum of the mean OD value of normal cell wells and 3 standard deviations is used as the critical value of positive reaction. When 99% virus being neutralized is regarded as the judgement criterion as same as it is in the classical mouse neutralization test (MNT), it appears that the method established could be suitable to replace MNT for the rabies neutralizing antibody assay. |
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| Keywords: | rabies virus Flury LEP strain neutralizing antibody micro-immunoen-zymatic test |
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