D-对羟基苯海因细菌的发酵工艺优化研究 |
| |
引用本文: | 贺莹,王晓冬,乔元彪.D-对羟基苯海因细菌的发酵工艺优化研究[J].安徽农学通报,2014(14):24-26. |
| |
作者姓名: | 贺莹 王晓冬 乔元彪 |
| |
作者单位: | 吕梁学院生命科学系,山西吕梁,033000;吕梁学院生命科学系,山西吕梁,033000;吕梁学院生命科学系,山西吕梁,033000 |
| |
基金项目: | 吕梁市科技攻关计划项目. |
| |
摘 要: | 通过筛菌得到一株野生菌 LLV-6,在诱导剂诱导后利用DL-对羟基苯海因(DL-HPH)为底物经D-海因酶(HYD)和N-氨甲酰-D-氨基酸酰胺水解酶(CAB)两酶水解而获得D-对羟基苯甘氨酸。用LB培养基(含磷酸盐缓冲溶液,pH=7.2)接菌量为0.5%(对数期的菌种),在37℃、200r、pH7.2条件下培养菌种。在培养12h后加入2%的诱导剂并以500μLDMSO作为促溶剂,33h后收集细胞。通过对细胞酶活力的测定,此时酶活力达到最大值,酶活力为0.57U/L,比优化前提高了2.6倍。
|
关 键 词: | D-对羟基苯甘氨酸 时间诱导 浓度诱导 酶活力 |
The Optimization of Fermentation Process of DL-Hydroxyphenyl Hydantoin Bacterial |
| |
Institution: | He Ying et al. (Department of Life Science, Lvliang College, Lvliang 033000, China) |
| |
Abstract: | Through the sieve bacteria get one natural bacteria LLV-6,In inducers induced effect after using the DL-Hydroxyphenyl Hydantoin(DL-HPH) as the substrate by D-hydantoinase(HYD)and N-carbamoylase (CAB)two enzyme hydrolysis of D-p-Hydroxyphenylglycine(D-HPG). Using LB culture medium(containing phosphate buffer solution,pH=7.2)the inoculation quantity was 0.5%(Logarithmic phase),the bacteria cul-tured at 37℃,200r,pH7.2 conditions. In 12 hours after adding 2% inducers and with DMSO as solvent,33 hours after collection of the cell. By measuring the enzyme activity of the cells,the enzyme activity reached a maximum value at this time,Enzyme activity was 0.57U/L,more than 2.6 times before optimiza-tion. |
| |
Keywords: | D-p-hydroxyphenylglycine Induction time Concentration induced Enzyme activity |
本文献已被 维普 万方数据 等数据库收录! |
|