Evaluation of Caspase-3 and Ki-67 expression in squamous cell hyperplasia of
the stomach induced by Platycodi radix water extract in Sprague–Dawley
rats |
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Authors: | Yinghua Li Seung-Beom Cha Youngil Park Bo-Ho Gong In-Yeong Jeong Hak-Soo Kim Min-Soo Kang Young-Suk Kim Chang Hoon Han Hyun-Kul Lee Si-Whan Song Chae-Gyoo Park Boo-Hyon Kang |
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Institution: | 1. Dt&SanoMedics. Co. Ltd., 15F, 126, Teheran-Ro, Gangnam-Gu, Seoul, Republic of Korea;2.Nonclinical Research Institute, ChemOn Inc., Yongin-Si, Republic of Korea;3.Non-clinical Center, Dt&CRO Co. Ltd., 46, Yurim-Ro 154 beon-Gil, Cheoin-Gu, Yongin-Si, Republic of Korea;4.Toxicology Lab, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Seoul, Republic of Korea |
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Abstract: | Platycodi radix is widely used in traditional herbal medicine for the treatment of bronchitis, asthma, pulmonary tuberculosis, hypertension, hyperlipidemia, and diabetes. This study aimed to investigate cell proliferation (Ki-67) and apoptosis (Caspase-3) potential in squamous cell hyperplasia of the stomach induced by a Platycodi radix water extract in a subchronic toxicity study. One hundred formalin-fixed, paraffin-embedded stomach tissues of rats treated with Platycodi radix at doses of 0, 500, 1,000, and 3,000 mg/kg body weight/day were used for the analysis. They were conventionally stained using hematoxylin and eosin (H&E) and immunohistochemically (IHC) stained using caspase-3 and Ki-67 antibodies. The incidence of squamous cell hyperplasia was significantly increased in the 3,000 mg/kg b.w./day treatment group in both sexes (p<0.01). However, the hyperplastic change was completely repaired after 4 weeks of recovery period. Ki-67 expression was similar in all groups, with no statistically significant differences among the groups. Caspase-3 expression was significantly increased in both sexes in the 3,000 mg/kg b.w./day treatment group (p<0.01), compared with the vehicle control groups, and then reduced to normal levels in the recovery groups in both sexes. In conclusion, this study showed that squamous cell hyperplasia induced by the Platycodi radix water extract in the limiting ridge of the stomach is not considered to be abnormal proliferative change; as a result, squamous cell hyperplasia is considered to be a non-adverse effect when induced by the oral administration of the Platycodi radix water extract once daily for 13 weeks in rats. |
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Keywords: | Caspase-3 Ki-67 antigen Sprague– Dawley rats squamous cell hyperplasia local irritation |
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