| 番茄子叶离体培养中激素的优化 |
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| 引用本文： | 李桂兰,乔亚科,武宝悦,蒲伟凤,王丹.番茄子叶离体培养中激素的优化[J].河北农业大学学报,2009,32(5). |
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| 作者姓名： | 李桂兰 乔亚科 武宝悦 蒲伟凤 王丹 |
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| 作者单位： | 河北科技师范学院,植物细胞工程实验室,河北,昌黎,066600 |
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| 基金项目： | 河北省自然基金项目，河北省科技厅科技领军人才项目，河北科技师范学院博士基金 |
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| 摘 要： | 为寻找在番茄离体培养中替代ZT的激素,本试验比较了6-BA不同质量浓度组合对‘中蔬五号’、‘中蔬六号’和‘黄色圣依’番茄愈伤组织的诱导及分化作用。结果表明:6-BA在1.0~3.0 mg/L范围内均可诱导愈伤组织的形成,以6-BA 3.0 mg/L最佳。番茄品种‘中蔬六号’出愈率及不定芽诱导率较高,其不定芽诱导率为76%以上。适时降低分化培养中基6-BA的浓度(0.5 mg/L),有利于愈伤组织正常不定芽的形成。
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| 关 键 词： | 番茄 组织培养 6-BA 再生 |
Optimization the hormones used in tomato cotyledon culture in vitro |
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| LI Gui-lan,QIAO Ya-ke,WU Bao-yue,PU Wei-feng,WANG Dan.Optimization the hormones used in tomato cotyledon culture in vitro[J].Journal of Agricultural University of Hebei,2009,32(5). |
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| Authors: | LI Gui-lan QIAO Ya-ke WU Bao-yue PU Wei-feng WANG Dan |
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| Institution: | LI Gui-lan,QIAO Ya-ke,WU Bao-yue,PU Wei-feng,WANG Dan(Plant Cell Engineering Lab.,Hebei Normal University of Science , Technology,Changli 066600,China) |
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| Abstract: | In order to search for the optimal hormone which alternate ZT in tomato cultured in vitro,the effects of media with different 6-BA concentrations on tomato varieties('zhongshu5','zhongahu6' and 'huangseshengyi')callus inducing,shoot differentiation were compared in the experiment.The results showed that callus could be induced in the media with 6-BA concentrations from 1.0 mg/L to 3.0 mg/L.The best medium was MS+NAA 0.05 mg/L+6-BA 3.0 mg/L,in which callus grew fast and many embryo callas were produced.the b... |
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| Keywords: | 6-BA |
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