小麦高分子量谷蛋白亚基1Ax 2*的AS-PCR鉴定

为了从DNA水平上快速鉴定Glu-A1位点编码的1Ax2^＊优质亚基，建立了稳定的检测1Ax2^＊优质亚基基因的PCR扩增体系。通过该体系，1Ax2^＊优质亚基基因扩增出2652bp特异片段，而其他亚基基因则不能扩增出该片段。验证材料的PCR鉴定结果与SDS-PAGE电泳结果一致，表明利用该体系鉴定1Ax2^＊亚基是可行的。利用此体系鉴定了44份外引小麦品种（系）的谷蛋白Glu-A1位点，有24个品种（系）含有1Ax2^＊亚基，1Ax2^＊亚基出现频率为54．5％。

Identification of 1Ax 2* Subunit of Wheat HMW Glutenin Using AS-PCR

In order to identify the 1Ax 2 subunit of high molecular weigh (HMW) glutenin coded by Glu-A1 locus at the DNA level, we constructed a stable AS-PCR amplified system of detecting 1Ax 2 subunit gene, which 1Ax 2 subunit gene could be amplified as a 2 652bp fragment, while other subunit genes on GluA1 locus could not be amplified. The result of AS-PCR identification was accordant with SDS-PAGE identification for testing varieties, showed that the AS-PCR system can be used in detecting of 1Ax 2 subunit. We used this AS-PCR method to detect 44 foreign wheat varieties(lines). The results showed that 24 varieties(lines)(54.5%) had 1Ax 2 subunit and 20 varieties(lines) had not.