| 高效制备转基因小鼠的优化试验 |
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| 引用本文： | 谢夏阳,滕勇,刘丽均,徐艺玫,唐一岷,徐平. 高效制备转基因小鼠的优化试验[J]. 上海交通大学学报(农业科学版), 2006, 24(1): 84-89 |
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| 作者姓名： | 谢夏阳 滕勇 刘丽均 徐艺玫 唐一岷 徐平 |
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| 作者单位： | 中国科学院,上海实验动物中心,上海,201615;中山大学,生命科学学院,广州,510275;第一军医大学,广州,510515 |
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| 摘 要： | 为了优化转基因小鼠的制备技术,试验着重对注射DNA的制备、高质量受精卵的获得、显微注射、胚胎移植及早期胚胎检测等各步骤中的主要影响因素进行了优化。移植后出生的小鼠经PCR及Southern Blot检测,阳性鼠检出率由1.85%(2/108)增加到23.36%(25/107)。由此说明,通过注射DNA的改良、技术优化以及GFP在转基因动物中的应用,转基因效率可以得到明显提高,为高效制作转基因动物提供基础。
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| 关 键 词： | 显微注射 胚胎移植 转基因小鼠 |
| 文章编号： | 1671-9964（2006）01-0084-06 |
| 收稿时间： | 2005-03-04 |
| 修稿时间： | 2005-03-04 |
Optimization Experiment to Establish Transgenic Mice in High Efficiency |
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| XIE Xia-yang,TENG Yong,LIU Li-jun,XU Yi-mei,TANG Yi-ming,XU Ping. Optimization Experiment to Establish Transgenic Mice in High Efficiency[J]. Journal of Shanghai Jiaotong University (Agricultural Science), 2006, 24(1): 84-89 |
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| Authors: | XIE Xia-yang TENG Yong LIU Li-jun XU Yi-mei TANG Yi-ming XU Ping |
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| Affiliation: | 1. Shanghai Lab Animal Center, CAS, Shanghai 201615;2. School of Life Science, Zhongshan University, Guangzhou 510275; 3. The First Military Medical University, Guangzhou 510515,China |
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| Abstract: | To optimize the technique of transgenic mice making,in this research,the major steps such as the preparation of DNA fragments for microinjection,the preparation of eggs with high quality,the microinjection and implantation and early embryos detection were optimized.The born mice were examined by PCR and Southern blot.The ratio of transgenic founder mice was improved from 1.85 %(2/108) to 23.36 %(25/107).Therefore,the technical platform for transgenic research in our center has been established due to the great improvement of the efficiency of producing transgenic mice by optimized techniques and application of GFP in transgenic animals. |
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| Keywords: | microinjection embryo implantation transgenic mice |
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