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| 玉米耐盐愈伤组织变异体的筛选及生理特性分析 | |
| 引用本文： | 韦小敏,王鹏,季良越,胡彦民.玉米耐盐愈伤组织变异体的筛选及生理特性分析[J].华北农学报,2007,22(3):137-140. |
| 作者姓名： | 韦小敏 王鹏 季良越 胡彦民 |
| 作者单位： | 1. 郑州牧业工程高等专科学校,生物工程系,河南,郑州,450011;河南农业大学,农学院,河南,郑州,450002 2. 郑州牧业工程高等专科学校,生物工程系,河南,郑州,450011 3. 河南农业大学,农学院,河南,郑州,450002 |
| 摘 要： | 用9个组合的玉米幼胚为外植体,诱导出胚性愈伤组织。继代5次后,用200 mmol/L NaCl和5.5 mmol/LHyp(羟基脯氨酸)连续筛选3次,获得耐盐愈伤组织变异体。试验结果表明:用Hyp筛选得到的愈伤组织,其分化频率高于用NaCl筛选的,Hyp的选择频率是NaCl的2倍;2种耐盐愈伤组织变异体中的可溶性糖含量和脯氨酸含量都高于对照,3种愈伤组织细胞的渗透势排序为:用Hyp筛选到的变异体<用NaCl筛选到的变异体<对照。 |
| 关 键 词： | 玉米 筛选 耐盐愈伤组织变异体 生理特性 |
| 文章编号： | 1000-7091（2007）03-0137-04 |
| 修稿时间： | 2006-10-24 |
Selection of Salt-tolerant Callus Variants and Analyses of their Physiological Characters in Maize |
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| WEI Xiao-min,WANG Peng,JI Liang-yue,HU Yan-min.Selection of Salt-tolerant Callus Variants and Analyses of their Physiological Characters in Maize[J].Acta Agriculturae Boreali-Sinica,2007,22(3):137-140. | |
| Authors: | WEI Xiao-min WANG Peng JI Liang-yue HU Yan-min |
| Institution: | 1. Department of Biology, Zhengzhou Animal Husbandry Engineering College, Zhengzhou 450011, China; 2. College of Agronomy, Henan Agricultural University, Zhengzhou 450002, China |
| Abstract: | The embryogenic calli were induced from immature embryos of 9 combinations of maizes.After 5 generations culture,salt-tolerant variants were obtained through three generations of continuous selection with 200 mmol/L NaCl and 5.5 mmol/L Hyp.The results showed that the differentiation frequency of calli selected by Hyp were two times as much as that by NaCl.The contents of soluble sugar and Proline in two variants were higher than in CK.The order of osmotic potential was: the variant selected with Hyp |
| Keywords: | Maize Selection Salt-tolerant callus variants Physiological characters |
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