云南甘蔗花叶病病原的初步鉴定

对发生在云南蔗区的甘蔗花叶病病原进行了初步鉴定。结果表明,其病原呈现复杂性,既有线状或球状病毒的单纯侵染,又有线状和球状两种病毒的复合侵染。电子显微镜检测发现:含线状病毒的样品中均可看到大量线状、略呈弯曲的病毒粒子,病毒粒子大多数长度约为750nm,具有典型的马铃薯Y病毒科病细胞病理特征:在细胞质内存在大量风轮状、卷筒状内含体。血清学鉴定17份样品,其中10个样品与马铃薯Y属病毒合成抗体呈阳性反应,根据马铃薯Y病毒科特异性简并引物设计对其中5个样品进行RT-PCR,扩增产物与预期结果一致,这表明病原属马铃薯Y属病毒;另7个样品则与马铃薯Y病毒属合成抗体呈阴性反应,表明它们所带病毒是马铃薯Y科病毒但不是马铃薯Y属病毒。鉴别寄主测定从各地采集的5个样品,在供试的13个甘蔗及其近缘属鉴别寄主上,其致病性存在明显差异,其中11号分离物侵染力最强,共侵染10个鉴别寄主,分布最广。

Preliminary Identification of Sugarcane Mosaic Pathogeny in Yunnan

Preliminary identification of sugarcane mosaic pathogeny was undertaken in Yunnan sugarcane planting regions, and the disease pathogeny infection occurred on complexity infecting by the simple linear or spherical virus or by the complex both of them: Lots of linear and crooked virus particles in 750nm length can be seen under the electron microscope in the sample containing linear virus, which had the same characters as Potyviridae virus: plentiful wheel-like and tome-like inclusion body in the cytoplasm; 17 samples were examined by serology, and there were 10 samples checked by antibody as a positive reaction, Carried out RT-PCR on 5 samples based on the special characters of Potyviridae virus primers, the amplification product was according with the anticipation, which made clear that the virus is a kind of Potyvirus virus; The other 7 samples were checked by antibody as a negative reaction, which proved that the virus carried with the Potyviridae virus and not to belong the Potyvirus virus. The virus on 13 sugarcane varieties or kindred section varieties from 5 samples collected universally, had evident differences in infection ability, the No.11 infected 10 sugarcane varieties, was the most serious and with the most extensively distribution.