影响桑子叶不定芽形成的因素

用正交设计法对影响桑子叶不定芽形成的诸因素进行了比较研究。结果得出：（１）诱导培养基采用ＭＳ无机盐加即维生素、６－苄氨基嘌呤３．０ｍｇ／Ｌ、吲跺乙酸０．３ｍｇ／Ｌ、果糖或葡萄糖３０ｇ／Ｌ、水解乳蛋白５００ｍｇ／Ｌ和琼脂粉６ｇ／Ｌ最为适合，不定芽诱导率达到８０％以上；（２）子叶初期培养中，光照以５００～１０００ｌｘ较为适宜；（３）预培养４～６ｄ的桑种子，其子叶不定芽诱导率较其余子叶为高。不定芽经继代培养后能在生根培养基中形成完整根系，移植到土壤后可成苗。

FACTORS INFLUENCING THE FORMATION OF ADVENTITIOUS BUDS FROM MULBERRY COTYLEDONS

The factors influencing the formation of adventitious buds were studied by means oforthogonal design.The results showed that,(1)A medium containing MS mineral salts,8p vitamins,3.0mg/l of 6-benzylaminopurine,0. 3mg/l of indole-3-acetic acid,30g/l offructose or glucose,500mg/l oflactalbumin hydrolysate,solidified with 6g/l of agarpowder was most favorable for inducing adventitous buds from mulberry cotyledons.With this medium,the induction rate reached more thn 80%,(2)During the initial cul-ture of the cotyledons,illumination at an intensity of 500-1000lx is desirable,(3)Cotyle-dons isolated from mulberry seeds that had undergone preculture mediurn and subse-quently rooted well in rooting medium.