| 校正的BO液培养法对牛精子体外获能的影响 |
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| 引用本文： | 阎志春,张天佑,梁裕利,王祖昆.校正的BO液培养法对牛精子体外获能的影响[J].华南农业大学学报,1991(Z1). |
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| 作者姓名： | 阎志春 张天佑 梁裕利 王祖昆 |
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| 作者单位： | 华南农业大学畜牧系
(阎志春，张天佑，梁裕利)，广东省农科院畜牧研究所(王祖昆) |
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| 基金项目： | 张天佑教授主持的国家自然科学基金 |
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| 摘 要： | 本试验校正了利用肝素和咖啡因使牛精子体外获能的传统的BO液配方及培养方法;利用去透明带仓鼠卵的穿透试验和顶体的台盼兰——姬姆萨双重染色技术检查了该方法对获能的影响,发现校正的BO液培养法缩短了预培养时间,增强了获能效果。
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| 关 键 词： | 牛精子 获能 去透明带仓鼠卵的穿透试验 台盼兰——姬姆萨顶体染色法 |
EFFECT OF A MODIFIED BO MEDIUM ON IN VITRO CAPACITATION OF BOVINE SPERMATOZOA |
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| Yan Zhichun Zhang Tianyou Liang Yuli.EFFECT OF A MODIFIED BO MEDIUM ON IN VITRO CAPACITATION OF BOVINE SPERMATOZOA[J].Journal of South China Agricultural University,1991(Z1). |
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| Authors: | Yan Zhichun Zhang Tianyou Liang Yuli |
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| Abstract: | The traditional BO medium and method using caffeine and heparin for the in vitro capacitation of bovine spermayozoa was modified in this study. Zona-free hamster eggs penetration test (HEPT) and Trypan blue-Giemsa stain technique were employed to examine the effect of mBO medium on capacitation and it was found that the new method shortened preincubation time as well as improved capacitation effect. |
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| Keywords: | Bovine spermatozoa Capacitation Zona-free hamster eggs penetration test(HEPT) Trypan blue-Giemsa stain |
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