| 一步法RT-PCR检测小西葫芦黄花叶病毒 |
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| 引用本文： | 张永江. 一步法RT-PCR检测小西葫芦黄花叶病毒[J]. 植物检疫, 2005, 19(3): 141-142 |
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| 作者姓名： | 张永江 |
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| 作者单位： | 中国检验检疫科学研究院动植物检疫研究所,北京,100024 |
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| 摘 要： | 本文在两步RT-PCR检测ZYMV的基础上进行了一步法RT-PCR检测小西葫芦黄花叶病毒(ZYMV)的实验,并对商品化一步法RT-PCR试剂盒和本文实验的一步法RT-PCR的效果和成本进行了比较,最终建立了一种比较实用的检测ZYMV的方法.
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| 关 键 词： | 一步法RT-PCR 小西葫芦黄花叶病毒 检测 |
Detection of Zucchini yellow mosaic virus by One-step RT-PCR |
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| Zhang Yongjiang. Detection of Zucchini yellow mosaic virus by One-step RT-PCR[J]. Plant Quarantine, 2005, 19(3): 141-142 |
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| Authors: | Zhang Yongjiang |
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| Abstract: | One-step RT-PCR detection of ZYMV was researched which based on two-step RT-PCR and compared it with Single step RT-PCR kit.The res ult displayed that the method was practical and can be used for reference in the detection of other viruses. |
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| Keywords: | one-step RT-PCR Zucchini yellow mosaic virus detection |
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