| 人心果原生质体分离初探 |
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| 引用本文： | 罗丽华,杨定海,林尤河,王业华.人心果原生质体分离初探[J].福建林业科技,2009,36(2). |
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| 作者姓名： | 罗丽华 杨定海 林尤河 王业华 |
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| 作者单位： | 海南大学园艺园林学院,海南,海口,570228 |
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| 摘 要： | 以人心果幼嫩叶片组织为材料,研究了酶液组合、酶解时间、酶液中甘露醇含量、pH值、温度等因素对其原生质体分离的影响,结果表明:以3%纤维素酶R-10+0.4%果胶酶Y-23为混合酶液,0.6 mol.L-1甘露醇为渗透压稳定剂,pH 5.6的酶液组合,在28℃的黑暗条件下振荡,酶解时间2 h,分离出的原生质体产量最佳,可达到12.4×106个.g-1。
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| 关 键 词： | 人心果 原生质体分离 原生质体产量 |
Preliminary Study on the Protoplasts Isolation of Manilkara zapota |
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| LUO Li-hua,YANG Ding-hai,LIN You-he,WANG Ye-hua.Preliminary Study on the Protoplasts Isolation of Manilkara zapota[J].Journal of Fujian Forestry Science and Technology,2009,36(2). |
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| Authors: | LUO Li-hua YANG Ding-hai LIN You-he WANG Ye-hua |
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| Institution: | LUO Li-hua,YANG Ding-hai,LIN You-he,WANG Yehua(Horticulture and Garden College of Hainan University,Haikou 570228,Hainan,China) |
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| Abstract: | The tender leaves of Manilkara zapota were used as experiment materials and the influences of enzyme solution combinations,zymohydrolysis time and mannitol concentration in the enzyme solution,pH value,and temperature on protoplast isolation were studied.The results showed that the highest yield(12.4×106/g) was obtained by treating the leaves with a mixture of 3% of cellulose enzyme R-10+0.4% of pectase Y-23 with pH value of 5.6 for 2h of zymohydrolysis time at the temperature of 28℃,and on the oscillation condition with 0.6 mol·L-1 of mannitol as the osmotic pressure stabilizer. |
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| Keywords: | Manilkara zapota protoplast isolation protoplast yield |
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