转基因生物新品种培育科技重大专项（2008ZX 080022003）；西北农林科技大学基本科研业务费专项资金（CX200903）；国家“863”计划项目（2011AA100501）。

为了明确农杆菌侵染普通小麦大龄幼胚对幼苗再生及生长发育的影响,以普通小麦基因型小偃22、西农1376、西农889为主区,农杆菌菌液浓度(OD600值分别为0、0.5、1.0、1.5)为副区,采用裂区设计,对农杆菌侵染大龄幼胚后幼苗再生及生长发育相关性状进行了测定。结果表明,不同小麦基因型对农杆菌侵染的敏感程度不同。不同浓度农杆菌侵染小麦大龄幼胚后,初期幼苗再生成苗率、鲜重、株高和根长并未随菌液浓度变化而变化;在幼苗生长发育过程中,随着农杆菌菌液浓度的提高,小麦幼苗叶绿素含量和SOD活性呈下降趋势,而幼苗POD活性、CAT活性和MDA含量呈上升趋势,且当菌液浓度大于等于1.0(OD600)时与对照呈现显著差异。综合农杆菌对再生幼苗生理生化指标的影响,认为当农杆菌菌液浓度达到1.0(OD600值)以上时将对幼苗的生长发育造成严重伤害。

Influence of Agrobacterium tumefaciens Infection on Large Immature Embryos of Wheat to Seedlings Regeneration and Growth

Large immature embryo of wheat genotypes of Xiaoyan 22, Xinong 1376 and Xinong 889 was used as main plot, Agrobacterium tumefaciens bacterial concentrations of 0, 0.5, 1.0 and 1.5 OD₆₀₀ was used as subplot, the influences of Agrobacterium tumefaciens infection on the regeneration of large immature embryos, the seedling growth and development related traits were studied using split plot design. The results showed that the sensitivity of different genotypes to the infection of Agrobacterium tumefaciens was different. The influence of Agrobacterium tumefaciens infection on the regeneration of wheat seedlings was not significant. The regeneration rate, fresh weight, plant height and root length of wheat seedling did not differe with the variation of bacterial concentration. However, during the growth and development process of seedling, the chlorophyll content and SOD activity of the seedlings showed a downward trend, and the POD activity, CAT activity and MDA content showed an increasing trend with the increasing of Agrobacterium tumefaciens bacterial concentration. It demonstrated significant difference with the control (0 OD₆₀₀) when the bacterial concentration reached 1.0 OD₆₀₀. In conclusion, when bacterial concentration reached 1.0 OD₆₀₀, Agrobacterium tumefaciens will significantly influence the growth and development of regenerated wheat seedlings.