| 1株产细菌素海洋乳酸菌的分离筛选及鉴定 |
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| 引用本文： | 赵鸭美,刘林,安静莹,胡雪琼,刘颖. 1株产细菌素海洋乳酸菌的分离筛选及鉴定[J]. 热带作物学报, 2013, 34(1): 171-175 |
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| 作者姓名： | 赵鸭美 刘林 安静莹 胡雪琼 刘颖 |
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| 作者单位： | 1. 广东省水产品加工与安全重点实验室 广东湛江524088;广东普通高等学校水产品深加工重点实验室 广东湛江524088;广东海洋大学食品科技学院 广东湛江524088
2. 贵州大学生命科学学院,贵州贵阳,550025 |
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| 基金项目： | 广东省科学技术厅项目“海洋微生物源天然食品防腐剂的筛选与应用”（No. 2010B020313002）。 |
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| 摘 要： | 采用牛津杯打孔法,以大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌、单核细胞增生李斯特菌、副溶血弧菌、米曲霉为指示菌,对从南海海域中捕捞的华贵栉孔扇贝肠道中分离到的9株乳酸菌进行抗菌活性测定,并对活性菌株的发酵液进行排酸、排过氧化氢,以及胰蛋白酶、木瓜蛋白酶、胃蛋白酶、蛋白酶K酶解实验,筛选出1株具有较强抑菌作用产细菌素的乳酸菌ZH-54.结合形态鉴定、生理生化特性实验以及16S rRNA序列同源性分析,鉴定菌株ZH-54为坚强肠球菌(Enterococcus durans),其16S rRNA在GeneBank的登录号为JX258806.
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| 关 键 词： | 乳酸菌 细菌素 抑菌试验 16S rRNA |
Screening and Identification of a Bacteriocin-producing Marine Lactic Acid Bacterium |
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| ZHAO Yamei,LIU Lin,AN Jingying,HU Xueqiong and LIU Ying. Screening and Identification of a Bacteriocin-producing Marine Lactic Acid Bacterium[J]. Chinese Journal of Tropical Crops, 2013, 34(1): 171-175 |
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| Authors: | ZHAO Yamei LIU Lin AN Jingying HU Xueqiong LIU Ying |
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| Affiliation: | Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, College of Food Science and Technology, Guangdong Ocean University;College of Life Science, Guizhou University;Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, College of Food Science and Technology, Guangdong Ocean University;Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, College of Food Science and Technology, Guangdong Ocean University;Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, College of Food Science and Technology, Guangdong Ocean University |
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