禽霍乱油乳剂灭活菌苗的研究

选用免疫原性好的５Ａ和７Ａ血清型巴氏杆菌菌株，分别接种于马丁肉汤通气培养。要求每ｍｌ的含菌量达１００亿以上。经０．５％福尔马林灭活后，等量混合为水相。１０＃白油为油相，按适当比例制成油乳剂灭活菌苗。与禽霍乱弱毒菌苗和禽霍乱氢氧化铝菌苗，分别接种于同群蛋鸡作对比试验。结果证实油乳剂灭活苗在安全性和免疫原性方面都优于后两种疫苗，５个月的攻毒保护率达８０％。经广泛的区域试用，从反馈信息和实地调查结果说明本菌苗对各种日龄的鸡、鸭、鹅都能有效地预防和控制禽霍乱的发生或流行。

IMMUNIZATION WITH THE OIL EMULSION VACCINE AGAINST FOWL CHOLERA

The 5∶A and 7∶A Pasteurella multocida strains with high immunogenicity were cultured in Martin broth respectively until the bacteria amount was up to 10 11 /ml. After inactivated by 0.5% formalin,the culture from both strains were mixed equally as water phase.Then,the oil emulsion vaccine was developed by usual methods through mixing the oil phase (10 # mineral pitch) and water phase in optimal proportion,which are more safe and higher immunogenic compared with the attenuated vacccine and aluminum hydroxide inactivated vaccine in laying hens. 80 percent protection rate of this vaccine was reached for five months. Through used in laying hens,