| 定点突变麻鸡副黏病毒ZH-1株F基因及其鉴定 |
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| 引用本文： | 李红丽,詹丽娥,王彩先,唐娟,陆冰洋. 定点突变麻鸡副黏病毒ZH-1株F基因及其鉴定[J]. 山西农业科学, 2011, 39(2): 164-166,173. DOI: 10.3969/j.issn.1002-2481.2011.02.21 |
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| 作者姓名： | 李红丽 詹丽娥 王彩先 唐娟 陆冰洋 |
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| 作者单位： | 山西省农业科学院畜牧兽医研究所,山西太原,030032 |
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| 基金项目： | 山西省自然科学基金资助项目(2008011072) |
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| 摘 要： | 根据麻鸡副黏病毒ZH-1株F基因序列,采用PCR体外定点突变技术,设计2对引物(引物中含有突变位点)。应用重叠PCR延伸法,分3次PCR扩增F基因,从而得到突变后的F基因,命名为F变基因,将其克隆进入pGEM-T载体,并进行PCR、酶切测序鉴定,使突变后的F变基因编码的氨基酸裂解位点为112 Gly-Arg-Gln-Gly-Arg-Leu117,具有弱毒株的特点。
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| 关 键 词： | 麻鸡 副黏病毒 F基因 定点突变 鉴定 |
Identification of the Point Mutation to F Gene of Avian Paramyxovirus Strain ZH-1 Isolated from Ma Chicken |
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| LI Hong-li,ZHAN Li-e,WANG Cai-xian,TANG Juan,LU Bing-yang. Identification of the Point Mutation to F Gene of Avian Paramyxovirus Strain ZH-1 Isolated from Ma Chicken[J]. Journal of Shanxi Agricultural Sciences, 2011, 39(2): 164-166,173. DOI: 10.3969/j.issn.1002-2481.2011.02.21 |
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| Authors: | LI Hong-li ZHAN Li-e WANG Cai-xian TANG Juan LU Bing-yang |
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| Affiliation: | LI Hong-li,ZHAN Li-e,WANG Cai-xian,TANG Juan,LU Bing-yang(Institute of Animal Husbandry and Veterinary Sciences,Shanxi Academy of Agricultural Sciences,Taiyuan 030032,China) |
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| Abstract: | Based on the published F gene sequences of avian paramyxovirus strain ZH-1 isolated from Ma chicken,two pairs of primers containing mutation sites were designed using PCR site-directed mutagenesis in vitro.Appling overlap extension PCR,F gene was PCR amplified for three times to get mutated F genes and then named as F variable genes.PCR products were cloned into pGEM-T Vector,PCR and the positive clone were confirmed by enzyme digestion,the reformed F mutated gene cleavage site amino acid sequence is 112 Gl... |
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| Keywords: | Ma chicken paramyxovirus F gene site-directed mutagenesis identification |
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