含6X小黑麦血缘的抗病小麦新材料的种子储藏蛋白分析

６Ｘ小黑麦品种Ｖｅｎｕｓ和２个普通小麦品种杂交回交，选育出ＮＲ９８４９等８个系群９４个系，其中大部份抗条锈病或（和）白粉病。应用酸性聚丙烯酰胺凝胶电脉（ＡＰＡＧＥ）分析了ＮＲ９８４９等系群的６４个株及其亲本Ｖｅｎｕｓ、小偃６号和川育１２号的醇溶蛋白，结果表明，５０．００％的株系具有１ＲＳ／１ＢＬ所特有的Ｇ１ｄ１Ｂ３位点，２０．３１％的株系在Ｇｌｉ－２位点发生了普通小麦与６Ｘ小黑麦遗传物质重组；６４个株系中出现了１种亲本类型，３种重组类型和３种突变重组类型，突变率１５．６２％。ＳＤＳ－聚丙烯酰胺凝胶电泳（ＳＤＳ－ＰＡＧＥ）指出，大多数株系Ｇｌｕ－１位点与普通小麦亲本相同，即由Ｇｌｕ－Ａ１编码的亚基１，Ｇｌｕ－Ｂ１编码的亚基７＋８、１４＋１５，Ｇｌｕ－Ｄ１编码的亚基５＋１０、２＋１２。文中还讨论了６Ｘ小黑麦向普通小

Seed Storage Protein Analysis of New Wheat Germplasm with Disease Resistance Derived from 6x Triticale

advance line sincluding NR9849 et. al. From 8 selected group were obtained from crosses and backcrosses between a hexaploid triticale variety and 2 common wheat. Most of these lines exhibited high resistance to wheat stripe rust and powdery mildew.Gliadin patterns of 64 lines and its parents including Venus,Xiaoyan 6 and Chuanyu 12 were analyzed by acid polyacrylamide gel electrophoresis (APAGE). The results showed that 50% of the lines contained G 1d 1B 3 loci conducted by 1RS/1BL of rye wheat translocation chromosome, and Gli 2 loci of genetic recombination between 6x triticale and wheat were existed in 20.31% lines, as well as 1 parent type, 3 recombinants type and 3 mutants type with mutation frequency of 15.62% were also observed in 64 lines. The HMW GS loci by SDS PAGE revealed that HMW glutenin subunits which were 1 code by Glu A 1 and 7 8 ,14 15 code by Glu B 1 and 5 10 ,2 12 code by Glu D 1 were uniform with its wheat parents in most of 64 lines. In addition,the breeding method of transfer genetic materials from 6x triticale into common wheat was discussed in present paper.