Analysis of chromosome-sized DNA and genome typing of isolated strains ofTaylorella equigenitalis |
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| Authors: | M Matsuda Y Asami T Miyazawa T Samata Y Isayama M Honda Y Ide |
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| Institution: | (1) Laboratory of Molecular Biology, Department of Biosciences, Azabu University, 1-17-71 Fuchinobe, 229 Sagamihara, Japan;(2) Department of Immunology, Azabu University, 1-17-71 Fuchinobe, 229 Sagamihara, Japan;(3) Department of Hygiene, College of Environmental Health, Azabu University, 1-17-71 Fuchinobe, 229 Sagamihara, Japan;(4) Faculty of General Education, Azabu University, 1-17-71 Fuchinobe, 229 Sagamihara, Japan |
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| Abstract: | Analysis of chromosome-sized DNA and genome typing ofTaylorella equigenitalis NCTC11184, Kentucky 188, and five strains ofT. equigenitalis isolated in Japan were carried out. The three restriction enzymes used,ApaI,NaeI andNotI, cleaved the genomic DNAs of five Japanese strains ofT. equigenitalis into relatively limited numbers of restriction fragments, which were well resolved on crossed-field gel electrophoresis (CFGE). The respective profiles after CFGE of the restriction fragments from all five strains were essentially identical to each other after digestion byApaI,NaeI orNotI. Hence it appears that these strains have a common genome type with respect to these three restriction enzymes. It was also shown that the respective profiles from these strains were essentially different from those ofT. equigenitalis NCTC11184 and those of Kentucky 188 after digestion withApaI,NaeI orNotI.Abbreviations CFGE
crossed-field gel electrophoresis
- FIGE
field inversion gel electrophoresis
- PFGE
pulsed-field gel electrophoresis |
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| Keywords: | chromosome-sized DNA electrophoresis genome typing Taylorella equigenitalis |
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