| 甘蔗抗旱生理测验及BADH基因PCR扩增的研究 |
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| 引用本文： | 曾华宗,郑成木.甘蔗抗旱生理测验及BADH基因PCR扩增的研究[J].热带作物学报,2003,24(1):55-58. |
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| 作者姓名： | 曾华宗 郑成木 |
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| 作者单位： | 华南热带农业大学农学院,海南,571737 |
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| 基金项目： | 国家自然科学基金(30060049)资助 |
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| 摘 要： | 对41份甘蔗种质材料进行了抗旱相关基因BADH(甜菜碱醛脱氢酶)基因PCR检测。结果显示，仅7份野生祖亲种种质材料及4份栽培品种材料有扩增带，且不同种质之间扩增带序列长度有差异。同时对30份甘蔗种质材料的离体叶片保水率、叶片相对含水量、气孔开度等水分生理指标进行测验，其结果与生产实际抗旱性表现基本一致。初步筛选出具有较强抗旱性生理和遗传基础的甘蔗祖亲种质和栽培品种种质材料。
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| 关 键 词： | 甘蔗 抗旱生理 BADH基因 抗旱基因 PCR 祖亲种质 栽培品种 生理测验 |
| 修稿时间： | 2002年6月28日 |
Physiological Test of Drought Resistance and PCR Amplification of BADH Gene in Sugarcane |
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| Zeng Huazong Zheng Chengmu.Physiological Test of Drought Resistance and PCR Amplification of BADH Gene in Sugarcane[J].Chinese Journal of Tropical Crops,2003,24(1):55-58. |
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| Authors: | Zeng Huazong Zheng Chengmu |
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| Abstract: | PCR detection of41accessions of sugarcane germplasms was made in terms of BADH(Betaine Aldehyde Dehydrogenase)gene related to drought resistance.Amplified bands were found in7accessions of ancestral species and4cultivar accessions,and these bands were different among germplasms.An attempt was made to study the physiological indexes of drought resistance of these sugarcane accessions,such as leaf water content after excision,leaf relative water content and stomatal aperture.The results conformed to their actual drought resistance in the field.Some accessions of these ancestral species and cultivars with stronger drought resistance were prelimi narily screened out as sugarcane breeding materials. |
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| Keywords: | sugarcane drought resistance -based gene PCR detection drought resistance characteristics physio logical test |
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