| 金黄色葡萄球菌TraP蛋白单克隆抗体制备 |
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| 引用本文： | 周雪,范志勇,汤炜,张丽萌,李闰婷,周瑜,崔玉东. 金黄色葡萄球菌TraP蛋白单克隆抗体制备[J]. 黑龙江八一农垦大学学报, 2012, 24(2): 48-51,99 |
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| 作者姓名： | 周雪 范志勇 汤炜 张丽萌 李闰婷 周瑜 崔玉东 |
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| 作者单位： | 1. 黑龙江八一农垦大学生命科学技术学院,大庆,163319
2. 大庆市第四中学 |
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| 摘 要： | 研究通过骨髓瘤细胞SP2／O与经金黄色葡萄球菌（S．aureus）Trap蛋白免疫的小鼠脾细胞融合，并进一步筛选种单克隆化，获得了11株稳定分泌抗TraP蛋白的单克隆抗体（McAb）杂交瘤细胞株，其中8株McAb（2A1、3A6、381、184、2C5、4C7、5C3和2D8）亚类为IgGl，3株McAb（2A7、3A1和1c4）亚类为IgM，轻链均为K链。8株IgGl型McAb的小鼠腹水的抗体效价均达到1：128000，交叉实验结果显示这8株McAb不与S．aureus的ClfA、ClfB、Cna、FnbPA和IsdB蛋白以及链球菌的GapC蛋白反应，具有良好的特异性，Western—blotting结果显示这8株McAb识别的都是TraP的线性表位。
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| 关 键 词： | 金黄色葡萄球菌 TraP 单克隆抗体 |
Preparation of Monoclonal antibodies against Staphylococcus aureus TraP |
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| Zhou Xue,Fan Zhiyong,Tang Wei,Zhang Limeng,Li Runting,Zhou Yu,Cui Yudong. Preparation of Monoclonal antibodies against Staphylococcus aureus TraP[J]. journal of heilongjiang bayi agricultural university, 2012, 24(2): 48-51,99 |
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| Authors: | Zhou Xue Fan Zhiyong Tang Wei Zhang Limeng Li Runting Zhou Yu Cui Yudong |
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| Affiliation: | 1(1.College of Life Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319;2.Daqing NO.4 Middle School) |
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| Abstract: | Eleven antibody-secreting hybridomas were produced in vitro by fusing myeloma cells line SP2/0 with spleen cells from a BALB/c mice immunized with Staphylococcus aureus Target of RNAIII-activiting protein(TraP).Eight(2A1、3A6、3B1、1B4、2C5、4C7、5C3 and 2D8)of these monoclonal anti-TraP antibodies were characterized as subtype IgG1 and κ chain,and the antibody titers of McAb produced by these eight hybridomas in ascites were 1∶128 000.These McAbs specific for TraP were identified by an indirect ELISA against protein ClfA、ClfB、Cna、FnbPA and IsdB from Staphylococcus aureus and GapC from Streptococcus.Results of western-blotting suggested that these McAbs were the lined epitopes of TraP. |
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| Keywords: | Staphylococcus aureus TraP monoclonal antibody |
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