| 罗汉果性别相关RAPD标记的克隆与序列分析 |
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| 引用本文： | 韦素玲,黄姿梅,杨华,秦新民.罗汉果性别相关RAPD标记的克隆与序列分析[J].湖北农业科学,2008,47(3):251-253. |
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| 作者姓名： | 韦素玲 黄姿梅 杨华 秦新民 |
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| 作者单位： | 广西师范大学生命科学学院,广西,桂林,541004 |
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| 摘 要： | 以罗汉果雌雄株叶片为材料,用90个随机引物进行筛选,其中S60和S90扩增得到3条雄株特异性片段.将这3条差异片段回收、克隆及测序,获得的序列分别为305bp、405bp和746bp.序列分析结果表明,这3个片段为罗汉果中新发现的DNA序列,与一些动物的性染色体有较高的同源性.该结果为进一步将RAPD标记转换成更稳定的SCAR标记,用于罗汉果早期性别的鉴别研究提供了条件.
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| 关 键 词： | 罗汉果 性别 RAPD 序列分析 |
| 文章编号： | 0439-8114(2008)03-0251-03 |
| 修稿时间： | 2007年12月10 |
Cloning and Sequence Analysis of the RAPD Markers Related to Sex in Siraidia grosvenorii |
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| WEI Su-ling,HUANG Zi-mei,YANG Hua,QIN Xin-min.Cloning and Sequence Analysis of the RAPD Markers Related to Sex in Siraidia grosvenorii[J].Hubei Agricultural Sciences,2008,47(3):251-253. |
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| Authors: | WEI Su-ling HUANG Zi-mei YANG Hua QIN Xin-min |
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| Institution: | WEI Su-ling,HUANG Zi-mei,YANG Hua,QIN Xin-min(College of Life Science,Guangxi Normal University,Guilin 541004,Guangxi,China) |
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| Abstract: | The molecular markers related to sex locus in Siraidia grosvenorii were screened by using RAPD technique with 90 random primers.3 male specific fragments were amplified by S60 and S90 random primers.305bp,405bp and 746bp sequences were identified through recycle,clone and sequence analysis.The results showed that the specific sequences were found for the first time in Siraidia grosvenorii,which shared a high homology in base sequences with the sex chromosome in animals.This study provided fundation for the ... |
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| Keywords: | Siraidia grosvenorii sex RAPD seqence analysis |
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