利用微卫星标记分析三个绵羊品种及杂交后代遗传多样性

利用微卫星标记技术,分析了无角陶赛特羊、滩羊、小尾寒羊及其杂交后代滩寒F1、陶滩寒F15个绵羊群体的遗传多样性,结果表明:7个微卫星位点均为高度多态位点,平均多态信息含量(PIC)达0.7077~0.8396;5个群体平均位点杂合度达0.7454~0.8665。以Nei氏遗传距离的UPGMA和NJ聚类结果表明,滩羊与滩寒F1具有较近的亲缘关系,小尾寒羊与陶滩寒F1具有较近的亲缘关系,可聚为一类;滩羊、小尾寒羊与陶赛特羊具有较远的亲缘关系。

Analysis of Genetic Diversity of Three Sheep Breeds and Two Crossbreeds by Microsatellite

The genetic diversity of three Sheep breeds and two crossbreeds was surveyed using 7 microsatellite loci. The five breeds included Polled Dorset Sheep, Tan Sheep,Small Tail Han Sheep,the Crossbreed of Tan Sheep and Small Tail Han Sheep, the Crossbreed of Polled Dorset Sheep and the Crossbreed of Tan Sheep and Small Tail Han Sheep. The 7 microsatellite loci showed high polymorphism information content value. The mean polymorphism information content value was between 0. 707 7- 0. 839 6,which -ndicated that the 5 breeds had abundant genetic diversity. The mean heterozygosity was between 0. 745 4 0. 866 5-In the UPGMA dendrogram and N J based on the Nei genetic distance, Tan Sheep and the Crossbreed of Tan Sheep and Small Tail Han Sheep had the intimate relationship; Small Tail Han Sheep and the Crossbreed of Polled Dorset Sheep and the Crossbreed of Tan Sheep and Small Tail Han Sheep had the close relationship, which were classified as one sort. But Tan Sheep and Small Tail Han Sheep had the distant relationship with Polled Dorset Sheep.