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| 以绿原酸为参照物的杜仲叶HPLC指纹图谱 | |
| 引用本文： | 左航,黄文,李天宏.以绿原酸为参照物的杜仲叶HPLC指纹图谱[J].安徽农业科学,2008,36(13):5482-5485. |
| 作者姓名： | 左航 黄文 李天宏 |
| 作者单位： | 1. 北京大学深圳研究生院城市人居环境科学与技术重点实验室,广东深圳,518065;北京大学环境工程系水沙科学教育部重点实验室,北京,100871 2. 北京大学环境工程系水沙科学教育部重点实验室,北京,100871;四川大学纳米生物医学技术与膜生物学研究所,四川大学华西医院肿瘤生物治疗国家重点实验室,四川成都,610041 3. 北京大学环境工程系水沙科学教育部重点实验室,北京,100871 |
| 摘 要： | 目的]用绿原酸为参照物建立杜仲叶的指纹图谱。方法]使用ZORBAX SB-C18(150 mm×4.6 mm,5μm)色谱柱,0.1%磷酸—乙腈梯度洗脱;流速1.0 ml/min;检测波长254 nm。结果]所得的色谱图各色谱峰分离较好,图谱参数符合《中药注射剂指纹图谱研究的技术要求(暂行)》的规定。结论]方法准确,可用于杜仲叶指纹图谱的建立以及控制杜仲叶药材的质量。 |
| 关 键 词： | 杜仲叶 绿原酸 高效液相色谱 指纹图谱 |
| 文章编号： | 0517-6611(2008)13-05482-03 |
| 修稿时间： | 2008年3月3日 |
HPLC Finger Print of Folium eucommiae with Chlorogenic Acid ad Reference Material |
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| ZUO Hang et al.HPLC Finger Print of Folium eucommiae with Chlorogenic Acid ad Reference Material[J].Journal of Anhui Agricultural Sciences,2008,36(13):5482-5485. | |
| Authors: | ZUO Hang |
| Abstract: | With chlorogenic acid as reference material,the fingerprint of Folium eucommiae was established by high-performance chromatography(HPLC).An analytical column of ZORBAX SB-C18(150 mm×4.6 mm,5 μm) was used to detect and the gradient elution was done by 0.1% acetontrile and the phosphoric acid with flow-rate of 1.0 ml/min and the column temperature of room temperature at the detection wave length of 254 nm.Every chromatographic peak of the chromatogram obtained was separated well and the parameter of the finger print in the research could meet with the stipulation in "Chinese Medicine Injection Fingerprint Technical Requirements(temporary)".The methods was accurate and suitable to establish finger print of Folium eucommiae by HPLC and control the quality of Folium eucommiae. |
| Keywords: | Folium eucommiae Chlorogenic acid High-performance chromatography Finger print |
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