<Emphasis Type="Italic">Agrobacterium</Emphasis>-mediated transformation of <Emphasis Type="Italic">Brachypodium distachyon</Emphasis> inbred line Bd21 with two binary vectors containing hygromycin resistance and GUS reporter genes |
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Authors: | Man Bo Lee Woong Bae Jeon Dae Yeon Kim Odgerel Bold Min Jeong Hong Yong Jin Lee Jae Han Park Yong Weon Seo |
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Institution: | 1.College of Life Sciences and Biotechnology,Korea University,Seoul,Korea |
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Abstract: | Brachypodium distachyon (Brachypodium) is a novel model plant for structural and functional genomic studies of temperate grasses. Brachypodium as a model plant has many favorable features, such as small size, small genome, short life cycle, self-fertility, and simple
growth requirements. The genome sequence of the standard line Bd21 has been released and genomic resources have been developed.It
is imperative to develop a method for efficient Agrobacterium-mediated Brachypodium transformation. Yellowish and compact embryogenic calli derived from immature embryos of the Bd21 were transformed with the
Agrobacterium strain AGL1. Seven- and nine-week-old calli were used for transformation with Agrobacterium carrying either pCAMBIA 1301 and pCAUGH. Transformation efficiencies were assessed through histochemical GUS assay. The efficiency
of transformation with pCAMBIA 1301 (based on the number of callus lines producing GUS-detected plantlets and the number of
calli used for transformation) reached 20.1% (7-week-old calli) and 1.7% (9-week-old calli), and with pCAUGH (based on the
number of GUS-detected plantlets and the number of regenerants) 90 and 87% for 7- and 9-week-old calli, respectively. High
selection pressure was obtained by using pCAUGH, which is preferred for saving labor and time consumption during the callus
selection. |
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