| 一种简单快速的拟南芥基因组DNA的微量提取方法 |
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| 引用本文： | 邹华文,黄丛林. 一种简单快速的拟南芥基因组DNA的微量提取方法[J]. 长江大学学报, 2006, 0(11) |
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| 作者姓名： | 邹华文 黄丛林 |
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| 作者单位： | [1]长江大学农学院 [2]北京市农林科学院北京农业生物技术研究中心 湖北荆州 |
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| 摘 要： | 报道了一种专供PCR分析的快速微量提取拟南芥基因组DNA的方法。该法大大简化了传统提取方法的步骤,而且用氯仿-异戊醇代替传统的酚,克服了由于酚的残留带来的后续PCR等操作上的困难。用该法提取的DNA样品质量较高,特别适合一些突变体及转基因植株的PCR初步鉴定。
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| 关 键 词： | DNA提取 拟南芥（Arabidopsisthaliana） PCR |
A Simple and Rapid Method for Genome DNA Extraction from Arabidopsis |
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| ZOU Hua-wen. A Simple and Rapid Method for Genome DNA Extraction from Arabidopsis[J]. Journal of Yangtze University, 2006, 0(11) |
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| Authors: | ZOU Hua-wen |
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| Abstract: | A simple and rapid method of extracting Arabidopsis genome DNA for PCR analysis was reported.The method simplified the traditional DNA extracting process.Furthermore,trichoromethane-isoamylol was used to replace hydroxybenzene.Consequently it overcame the difficulties of PCR analysis resulted from rudimental hydroxybenzene.The DNA extracted via this method provided higher quality and is suitable for PCR analysis of some mutants and transgenic plants. |
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| Keywords: | DNA extraction Arabidopsis PCR |
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