分月扇舟蛾颗粒体病毒生物测定

从黑龙江省双城林场采集健康的分月扇舟蛾2龄幼虫,将室内保存一年的分月扇舟蛾虫尸研碎,分离提纯得到纯净的颗粒体病毒。将病毒悬液稀释为1..58×103PIB·mL-1,1.58×104PIB·mL-1,1.58×105PIB·mL-1,1.58×106PIB·mL-1,1.58×107PIB·mL-1,1.58×108PIB·mL-1,1.58×109PIB·mL-17种浓度,采集新鲜的杨树叶分别浸入上述不同浓度的悬液,对采集的健康幼虫进行接毒。9天实验结果表明,浓度为1..58×103PIB·mL-1时校正死亡率最小(7.32%),浓度是1.58×109PIB·mL-1时校正死亡率最大(97.36%),浓度对数和死亡几率值的回归直线方程为y=1.946 0.558x,LC50为2.97×105PIB·mL-1。1.58×105PIB·mL-1,1.58×106PIB·mL-1,1.58×107PIB·mL-1,1.58×108PIB·mL-1,1.58×109PIB·mL-1w所对应的半致死时间(LT50)分别为8.55d,6.89d,5.9d,4.65d,4.08d。随着浓度的增大,LT50逐渐缩短,说明该病毒的毒力较强,作为杀虫剂具有很好的应用潜力。

Bioassay of Clostera anastomosis granulosis virus

The second-instar healthy larvae of Clostera anastomosis were collected in the artificial woodland of poplar in Shuangcheng Town, Heilongjiang Province, China. The dead larvae of C. anastomosis infected by granulosis virus (GV) of Clostera anastomosis were grinded to obtain GV. The GV viral pesticide was diluted to seven concentrations, 1.58×10³PIB·mL⁻¹, 1.58×10⁴ PIB·mL⁻¹, 1.58×10⁵ PIB·mL⁻¹, 1.58×10⁶ PIB·mL⁻¹, 1.58×10⁷ PIB·mL⁻¹, 1.58×10⁸ PIB·mL⁻¹ and 1.58×10⁹ PIB·mL⁻¹ and the fresh poplar leaves were dipped in the seven concentrations liquids to feed the larvae. After nine days the mortality of larvae was investigated. The minimum corrected mortality (7.32%) of larvae was observed at concentrations of 1.58×10³PIB·mL⁻¹ and the maximal mortality (97.36%) was observed at concentration of 1.58×10⁹ PIB·mL⁻¹. The regression equation between the logarithm of the virus concentration and the mortality was y=1.946+0.558x. The LC₅₀ was 2.97×10⁵PIB·mL⁻¹. The LT₅₀ for the virus concentration of 1.58×10⁵, 1.58×10⁶, 1.58×10⁷, 1.58×10⁸, 1.58×10⁹ PIB·mL⁻¹ were 8.55d, 6.89d, 5.9d, 4.65d, and 4.08d, respectively, shorting gradually with the concentration increasing. It is concluded that the toxicity of Clostera anastomosis GV is very strong and as a kind of insecticides it has big potential in practical application. Biography: LI Hai-xia (1980–), female, Master degree student in Heilongjiang Forest Research Institute, Harping Rd., Harbin, 150040, P. R. China.