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| 表达ETEC F41的干酪乳杆菌免疫小鼠后的黏膜免疫应答 | |
| 引用本文： | 刘建奎,魏春华,余丽芸,何晓杰,赵志刚,张宁,刘双翼. 表达ETEC F41的干酪乳杆菌免疫小鼠后的黏膜免疫应答[J]. 动物医学进展, 2009, 30(3) |
| 作者姓名： | 刘建奎 魏春华 余丽芸 何晓杰 赵志刚 张宁 刘双翼 |
| 作者单位： | 1. 黑龙江八一农垦大学动物科技学院,黑龙江大庆,163319 2. 黑龙江八一农垦大学动物科技学院,黑龙江大庆,163319;黑龙江八一农垦大学生命科技学院,黑龙江大庆,163319 3. 黑龙江八一农垦大学生命科技学院,黑龙江大庆,163319 |
| 基金项目： | 黑龙江省教育厅课题，大庆市攻关课题，研究生创新工程项目 |
| 摘 要： | 将SPF级Balb/c小鼠随机分为免疫组和对照组,免疫组小鼠以pLA-F41/L.casei重组干酪乳杆菌滴鼻免疫,对照组用pLA/L.casei干酪乳杆菌滴鼻免疫,应用间接ELISA测定血清中特异性IgG抗体水平和鼻咽部冲洗液、肠道冲洗液、阴道冲洗液及粪便中ETEC F41特异性SIgA抗体水平.分离并计数NALT、NC及PP、IEL淋巴细胞数目,利用免疫细胞化学法检测CD4+、CD8+T细胞亚群水平,观察重组干酪乳杆菌滴鼻免疫小鼠诱导的鼻相关淋巴组织(NALT)和肠相关淋巴组织(GALT)黏膜部位的免疫应答.结果表明重组干酪乳杆菌滴鼻免疫小鼠可有效诱导NALT和GALT黏膜部位免疫应答. |
| 关 键 词： | 产肠毒素性大肠埃希菌 滴鼻免疫 鼻相关淋巴组织 肠相关淋巴组织 |
Mucosal Immune Response after Intranasal Immunization with Lactobacillus casei Expressing ETEC F41 |
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| LIU Jian-kui,WEI Chun-hua,YU Li-yun,HE Xiao-jie,ZHAO Zhi-gang,ZHANG Ning,LIU Shuang-yi. Mucosal Immune Response after Intranasal Immunization with Lactobacillus casei Expressing ETEC F41[J]. Progress In Veterinary Medicine, 2009, 30(3) | |
| Authors: | LIU Jian-kui WEI Chun-hua YU Li-yun HE Xiao-jie ZHAO Zhi-gang ZHANG Ning LIU Shuang-yi |
| Affiliation: | 1.College of Animal Science and Technology;Heilongjiang August First Land Reclamation University;Daqing;Heilongjiang;163319;China;2.College of Life Science and Technology;China |
| Abstract: | SPF Balb/c mice were randomly divided into two groups, mice in experiment group were intranasally immunized(i.n) with recombinant pLA-F41/L.case, while the control mice were given pLA/L.casei. Specific anti-F41 immunoglobulin G (IgG) antibody and specific anti-F41 secret immunoglobulin A (sIgA) antibodies in the lungs,intestines,vagina fluids and feces of mice were detected by indirect enzyme linked immunosorbent assay (ELISA) after intranasal immunization.Lymphocytes in NALT,NC,PP and IEL were isolated and... |
| Keywords: | ETEC F41 intranasal immunization NALT GALT |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |