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| 鹅细小病毒QY株的生物特性研究 | |
| 引用本文： | 李惠敏,李福伟,张桂红. 鹅细小病毒QY株的生物特性研究[J]. 水禽世界, 2011, 0(1): 32-35 |
| 作者姓名： | 李惠敏 李福伟 张桂红 |
| 作者单位： | 1. 山东省农业科学院家禽研究所,山东,济南,250023 2. 山东省农业科学院家禽研究所,山东,济南,250023;山东省家禽育种工程技术研究中心 3. 华南农业大学兽医学院 |
| 摘 要： | 本研究的病毒是从广东患病鹅的小肠组织中分离的,初步鉴定为小鹅瘟病毒(Goose parvovirus,GPV),命名为QY/05后,对病毒的生物学特性进行了鉴定。病料经过处理后,接种12日龄非免疫鹅胚,传代,发现病毒对鹅胚的平均致死时间为3～4d,胚体全身出现较为严重的出血点。病毒接种鹅成纤维细胞和番鸭成纤维细胞,显微镜下可见细胞圆缩、脱落、折光性增强等明显的细胞病变。鹅胚尿囊液经梯度离心后,磷钨酸负染,电镜下可见明显细小的病毒样粒子。这株小鹅瘟病毒尿囊液对鹅胚的EID50为10-3.67/0.3ml。对鹅胚成纤维细胞的TCID50分别为2-5.425/0.1ml。感染一个病毒最小致死量的鹅胚平均死亡时间(MDT)分别为96h。提取DNA后,PCR扩增,在阳性对照处扩增出需要的目的条带。研究结果表明,该分离毒株为典型的鹅细小病毒(Goose Parvovirus,GPV)。 |
| 关 键 词： | 鹅细小病毒 生物学特性 |
Study on biological characteristics of goose parvovirus QY strain |
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| LI Hui-min,LI Fu-wei,ZHANG Gui-hong. Study on biological characteristics of goose parvovirus QY strain[J]. Poultry Science, 2011, 0(1): 32-35 | |
| Authors: | LI Hui-min LI Fu-wei ZHANG Gui-hong |
| Affiliation: | LI Hui-min1,LI Fu-wei1,2,ZHANG Gui-hong3 (1.Institute of Poultry Shandong Academy of Agricultural Sciences Jinan,P.R China,250023,2.Poultry Breeding Engineering Technology Center of Shandong Province Shandong,3.College of Veterinary Medicine,South China Agricultural University,Guangzhou,) |
| Abstract: | One strain of goose parvovirus was isolated from intestines samples of infected goose in Guangdong areas. The strain was designated as QY/05. Biological characteristics of the viruse was studied by virus pathogenicity to goose embryos, morphological observation by electron microsope, EID50 to 12-day-old goose embryos and virus inoculation to 12-day-old goslings. It was found that the medium death time of goose embryos was about 3-4 days. There were hemorrhage points on the head, neck and back of the dead em... |
| Keywords: | goose parvovirus biological characteristics |
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