鸡品种随机引物扩增多态DNA的研究

用12个随机引物对6个鸡品种60个个体核DNA进行了RAPD扩增,共检出223条扩增片断,多态性片断188条,占843%。反映了品种内和品种间的遗传变异。12个引物对60个个体分别扩增出16,21,22,17,17,20,22,12,18,23,18,16条带。品种内和品种间遗传相似度变异范围为07981～08685和05993～07813。肉鸡AA品种内变异程度最大,且与伊莎褐遗传距离最大为04007。海兰白和海赛克斯褐首先聚类。肉鸡AA在UPGMA法中与海兰褐聚类后再与其它4种蛋鸡聚类。在NJ法中与海兰褐和其它4种蛋鸡的聚合同时聚类。说明了肉鸡AA与5种蛋鸡品种的距离较远。12个引物在6个鸡品种共检测到7条品种特异带和3条与性别相关带。

STUDY OF RAPD ON FOWLS BREEDS

Random amplified polymorphic DNA(RAPD)technique was applied to assess the genetic variations in six breeds with 12 random primer given 223 reproducible amplified fragements.The polymorphic fragement was 188 bands(84 3%),that reflected genetic variations in breeds and among breeds.Amplification with 12 primer checked out 16,21,22,22,17,17,20,22,12,18,23,18 and 16 bands for sixty individuals. The scope of genetic similarity variations was 0 798 1～ 0 868 5 in six fowls breeds 0 599 3 ～ 0 781 3 among six fowl breeds.The variations was the biggest in Abor Acres.The genetic distance was 0 4007 between Abor Acres and ISA Brow. The cluster analysis indicated a distant relationship between Abor Acres and five egg line fowl breeds.As the same time,amplification with 12 primer given 7 special bands and 3 bands was interrelated with sex.