| 人工喂养幼虎体内细小病毒的分离与序列分析 |
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| 引用本文： | 刘丹,高玉伟,杨松涛,王立刚,王淑君,侯小强,刘玉秀,王伟,周明,夏咸柱.人工喂养幼虎体内细小病毒的分离与序列分析[J].安徽农业科学,2010,38(15):7855-7856,7877. |
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| 作者姓名： | 刘丹 高玉伟 杨松涛 王立刚 王淑君 侯小强 刘玉秀 王伟 周明 夏咸柱 |
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| 作者单位： | 1. 黑龙江东北虎林园,黑龙江哈尔滨,150028
2. 军事医学科学院军事兽医研究所,吉林长春,130062 |
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| 基金项目： | 国家林业局野生动植物保护司资助项目 |
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| 摘 要： | 目的]鉴定人工喂养幼虎的病原及其序列特征。方法]应用FPV特异引物对8只哈尔滨虎园人工喂养幼虎的肺组织进行PCR检测,应用猫肾细胞（F81）细胞对PCR检测阳性样品进行病毒分离,并进行序列分析。结果]从8份样品中均扩增到761bp的核酸片段。进而应用F81细胞从其中的1份样品中分离获得1株猫细小病毒。VP2序列分析表明,该病毒与此前在该虎园分离获得的虎源猫细小病毒同源性高达99.8%。结论]猫细小病毒对未接受母乳喂养的幼虎威胁严重,应加强对人工喂养幼虎的被动免疫预防。
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| 关 键 词： | 虎 细小病毒 病毒分离 序列分析 |
Isolation and Sequence Analysis of Parvovirus in Artificial Feeding Newly Born Tigers |
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| LIU Dan et al.Isolation and Sequence Analysis of Parvovirus in Artificial Feeding Newly Born Tigers[J].Journal of Anhui Agricultural Sciences,2010,38(15):7855-7856,7877. |
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| Authors: | LIU Dan |
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| Institution: | LIU Dan et al(Harbin Siberian Tiger Zoo,Harbin,Heilongjiang 150028) |
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| Abstract: | Objective] To identify parvovirus and analyze its sequence in artificial feeding newly born tigers.Method] Eight lung tissue samples of artificial feeding newly born tigers were detected by PCR method with feline parvovirus(FPV)specific primers.The isolation of FPV from positive sample was performed with F81 cell lines and the sequence of VP2 gene was then analyzed.Result] A positive 761 bp DNA fragment was found in all the lung tissues.A feline parvovirus strain HT-410 was isolated from 1of 8 samples by... |
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| Keywords: | Tiger Parvovirus Virus isolation Sequence analysis |
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