春荷鼎杂交根状茎的组培快繁的研究

摘要：【研究目的】本文采用传统品种春荷鼎与普通春兰杂交的继代根状茎为试材，对根状茎的增殖和分化进行研究。【方法】探讨不同培养基、转换培养基、6-BA与NAA、IBA对根状茎增殖、分化的影响。【结果】不同培养基对生长率有明显影响，1/2 MS为春兰根状茎的基本培养基；最适增殖培养基为1/2 MS+BA0.1+NAA0.1；根状茎的最佳分化培养基为1/2 MS+BA1.0+IBA1.0；以1/2 MS+BA1.0+IBA1.0培养基再转入B5+BA1.0+IBA1.0培养得交替培养方式，获得分化率达76%。【结论】组培快繁是培育春兰新品种的重要技术手段。

Study of the rootstocks about Cymbidium faberi ’ chun he ding’ in Rapid Multiplication Techniques

[OBJECTIVE]Taking the rootstocks of Cymbidium goeringii as test pieces, Cymbidium faberi ＂ chun he ding＇ and common Cymbidium ~aberi were employ as parents, study on Multiplication and differentiation of rhizomes in vitro of Cymbidium goeringii. [METHOD] Four factors of medium ,6-BA and NAA, IBA were design to study the effects on differentiation of rhizome. [RESULTS] The result show that the growth rate was affected evidently by media, The 1/2 MS was used as basal medium, The optimal Multiplication Conditions For the rootstocks were that 1/2MS ＋BA0.1 ＋NAA0.1, The best differentiation medium wasl/2 MS＋ BA1.0＋IBA1.0,Firstly using 1/2MS ＋ BA1.0 ＋ IBA1.0 and then using B5 ＋ BAI.0 ＋IBAI.0 could gain a higher differentiation rate of the rootstocks to 76 Percentage. [CONCLUSION]Rapid Multiplication Techniques were Cultivation Cymbidium fabed variety import technological means.