卷烟烟气及绿茶对蚕豆根尖细胞微核的诱导作用

目的]研究不同类型卷烟烟气水溶物对蚕豆（ViciafabaL.）根尖细胞微核的诱变作用,并在此基础上探讨了绿茶添加剂对卷烟烟气诱导微核的抑制作用。方法]卷烟品牌分别为白沙、中南海和狮牌,类型分别为烤烟型、混合型和雪茄型。烟气水溶物的浓度分别为1/8母液、1/4母液和1/2母液,烟气茶溶物浓度为1/4母液。结果]烟气水溶物能够引起蚕豆根尖细胞微核率发生明显变化,并在一定范围内随浓度增强而加强,其中,狮牌〉白沙〉中南海。相同浓度下,各类型卷烟烟气茶溶物引起的细胞微核率低于同种卷烟烟气水溶物。绿茶对狮牌卷烟微核诱导的抑制作用最明显,对白沙次之,对中南海卷烟最弱。结论]该研究可为将绿茶用作添加物从而降低烟毒的可行性提供科学依据。

Mutagenesis of Cigarette Smoke and Green Tea on Vicia faba L. Root Tip Cells

Objective] The research aimed to study the micronucleus mutagenesis of different types of cigarette smoke aqueous extract on root tip cells of Vicia faba L.,and based on this,the inhibitory action of green additives on cigarette smoke induced micronuclei in vitro was discussed.Method] The cigarette brand were Baisha,Zhongnanhai and Shipai,and the cigarette type was flue-cured type,mixed type and cigar type respectively.The aqueous extracts of smoke was one eighth of the mother liquor concentration,a quarter of mother liquor concentration and half the concentration of mother liquor respectively,and the tea extracts of smoke was a quarter of mother liquor.Result] The results showed that the aqueous extracts of smoke could cause root tip cells of Vicia faba L.micronucleus change significantly,and within a certain range,the micronucleus rate strengthened with the concentration increase,thereinto,Shipai>Baisha>Zhongnanhai.Under the same concentration,the micronucleus rate caused by different type of tea extracts of smoke was lower than that of aqueous extracts of smoke.The inhibitory action of green tea on micronucleus mutagenesis of cigarette was as follow:Shipai> Baisha> Zhongnanhai.Conclusion] The study can provide scientific basis for the feasibility of reducing tobacco and drugs by adding green tea.