冬藏对宁夏枸杞插穗内含物影响研究

为了探讨冬藏对插穗内含物的影响,寻找宁夏枸杞扦插的适宜采条时期,为宁夏枸杞扦插育苗提供理论依据,该研究设冬藏与留树两种处理,并研究不同处理对可溶性糖、淀粉、可溶性蛋白质、游离脯氨酸、过氧化物酶(POD)、多酚氧化酶(PPO)、吲哚乙酸氧化酶(IAAO)、单宁等8个内含物指标的影响。研究表明:冬藏会降低插穗内的游离脯氨酸、可溶性糖、淀粉、蛋白质等的含量和POD、PPO、IAAO等酶的活性,增加单宁含量。因而会降低插穗的成活率和新梢生长量。插穗留树有利于插穗的成活和新梢快速生长。研究表明宁夏枸杞硬枝扦插适宜的采条时期是春季萌动前。

Research on the Effect of Winter-Storing on Cuttings Inclusion Concentration of Lycium barbarum

Abstracts:In order to study the effect of cuttings inclusion of Lycium barbarum which were winter stored and find out a appropriate period for collecting its cuttings and provide the theoretical basis for cutting propagation of Lycium barbarum,we set up an experiment on the determination of eight different kinds of inclusion indicators such as soluble sugar,starch,soluble protein,free proline,peroxidase (POD),polyphenol oxidase (PPO),indole acetic acid oxidase (IAAO),and tannin in the cuttings treated by the methods of on tree storage and winter storing.The results showed that winter storing treatment could strongly reduced the content of free proline,soluble sugar,starch and protein and the activity of POD,PPO,IAAO and other enzymes,while increased the tannin content,which means winter storing would reduce the survival rate of cuttings and the length of its new shoot.In contrast,on tree storage was beneficial to the cuttings survival and the rapid growth of new shoots.This revealed that the more suitable period for collecting is in the spring before germinating.