生物频谱对牛胚胎体外生产效率的影响

本试验利用可调式周林生物频谱发生器对牛卵母细胞体外成熟、体外受精和体外培养过程实施辐射 ,以提高牛胚胎体外生产效率。频谱发生器与培养材料的距离为 5cm ,辐射强度根据辐射温度加以调节。实验结果表明 ,辐射温度控制在 39℃时 ,入孵卵母细胞的卵裂率 ( 63% )与对照组 ( 61 % )无显著差异(P >0 0 5) ,而囊胚发育率 ( 39% )显著高于对照组 ( 1 9% ) (P <0 0 5) ;当辐射温度达到 4 0℃时 ,实验组的卵裂率仅为 1 4 % ,囊胚率为 0。体外受精生产的囊胚用EFS4 0液进行玻璃化冷冻保存 ,解冻后发现 ,整个生产过程经生物频谱辐射胚胎的体外发育率 ( 86% )显著高于对照组 ( 65% )。

Effects of Bio-Frequency Spectrum Irradiation on the Efficiency of Bovine Embryo Production In Vitro

This experiment was to study effects of Biofrequency Spectrum(BFS) irradiation during in vitro fertilization on bovine embryo production in vitro.The distance from the BFS apparatus to the oocytes or embryos was 5cm during irradiation,and the irradiation intensity was adjusted in order to keep the irradiating temperature at 39℃ or 40℃.The results showed that the rates of cleavages existed no significant difference between the irradiation and the control group when the irradiating temperature was kept at 39℃(P>0 05),however,the blastocysts rate in the irradiation group was significantly higher than the control(P<0 05).When the irradiating temperature was 40℃,both rates of cleavages and blastocysts in the irradiation group were greatly significantly lower than the control(P<0 01).The early blastocysts from in vitro production with and without BFS irradiation were cryopreserved by vitrification in EFS40 solution respectively,the developmental rate of the blastocysts with BFS irradiation was significant higher than the without BFS irradiation(P<0 05)