| 富含多糖·多酚植物组织RNA的提取 |
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| 引用本文： | 王文锋,穆灵敏,张光谋. 富含多糖·多酚植物组织RNA的提取[J]. 安徽农业科学, 2009, 37(12): 5383-5383 |
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| 作者姓名： | 王文锋 穆灵敏 张光谋 |
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| 作者单位： | 新乡医学院生命科学技术系,河南新乡,453003;新乡医学院形态学实验室,河南新乡,453003 |
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| 基金项目： | 河南省科技厅2007年立项课题(072300450330)资助 |
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| 摘 要： | [目的]介绍一种适合于富含多糖、多酚植物材料的RNA提取方法。[方法]采用改进的异硫氢酸胍法提取植物材料的RNA，用紫外分光光度计测定其浓度和纯度，并通过RT-PCR进一步检测其质量。[结果]提取总RNA中的28、18、5s条带清晰，比例适中，OD260nm/OD280nm。为1．8—2．0。以提取的总RNA反转录合成的cDNA为模板，能扩增出预期长度的片段，无非特异性产物。[结论]该方法简便易行，成本较低，适合于富舍多糖、多酚植物材料的RNA提取。
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| 关 键 词： | 多糖 多酚 RNA提取 |
RNA Extraction of Plant Materials Rich in Polysaccharide and Polyphenol |
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| WANG Wen-feng et al. RNA Extraction of Plant Materials Rich in Polysaccharide and Polyphenol[J]. Journal of Anhui Agricultural Sciences, 2009, 37(12): 5383-5383 |
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| Authors: | WANG Wen-feng et al |
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| Affiliation: | WANG Wen-feng et al(Department of Life Sciences , Technology,Xinxiang Medical College,Xinxiang,Henan 453003) |
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| Abstract: | [Objective] The study aimed to introduce a kind of method on extracting RNA from plant materials rich in polysaccharide and polyphenol.[Method] The improved guanidine isothiocyanate method was used to extract RNA from plant,and the purity was used to determine with UV spectrophotometer.And its quality was further determined through RT-PCR.[Result] The 28,18 and 5 S bands in the RNA extracted had clear bands and proper proportion,with OD260 nm/OD280 nm of 1.8-2.0.Taking the synthesized cDNA of the extracted ... |
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| Keywords: | Polysaccharide Polyphenol RNA extraction |
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