| 野生欧洲李组织培养技术 |
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| 引用本文： | 耿文娟,吴玉霞,袁海英,廖康,周龙,许正. 野生欧洲李组织培养技术[J]. 经济林研究, 2009, 27(1) |
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| 作者姓名： | 耿文娟 吴玉霞 袁海英 廖康 周龙 许正 |
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| 作者单位： | 新疆农业大学,园艺学院,新疆,乌鲁木齐,830052;新疆伊犁园艺技术推广总站,新疆,伊宁,835000 |
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| 基金项目： | 国家自然科学基金，教育部高等学校博士学科点专项科研基金 |
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| 摘 要： | 以濒危果树野生欧洲李当年生新梢为外植体,进行组织培养研究.以期筛选出最优培养基配方及培养程序.结果表明:野生欧洲李初代培养时,以Bs为基本培养基优于MS培养基;培养基中添加一定量激素的外植体生长势明显优于不添加激素;以B5为基本培养基,6-BA质量浓度0.5～1.0 rag/L,并添加一定量的生长素(IBA、NAA)效果较优,能较快启动生长反应,并初步形成一定的增殖率;芽增殖培养阶段,6-BA质量浓度为0.5～I.0mg/L时,以NAA为生长素,NAA和6-BA适宜的比例为1·(1-3),以IBA为生长素,IBA和6-BA适宜的比例为1:(3～5)较适宜.
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| 关 键 词： | 生物工程 野生欧洲李 组织培养 激素 |
Tissue Culture Techniques of Prunus domestica L. |
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| GENG Wen-juan,WU Yu-xia,YUAN Hai-ying,LIAO Kang,ZHOU Long,XU Zheng. Tissue Culture Techniques of Prunus domestica L.[J]. Economic Forest Researches, 2009, 27(1) |
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| Authors: | GENG Wen-juan WU Yu-xia YUAN Hai-ying LIAO Kang ZHOU Long XU Zheng |
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| Affiliation: | 1.Horticulture College;Xinjiang Agricultural University;Urumqi 830052;Xinjiang;China;2.Horticulture Techniques Extension Center of Yili;Yining 835000;China |
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| Abstract: | In order to select out optimum media formula and culture program,tissue culture techniques of wild European plum(Prunus domestica L.) were researched,which belong to endangered fruit tree.The results showed that B5 basic medium was better than MS medium in initial culture;Explants had better growth vigor in the media supplemented with some hormones than that in the media without any hormone;Explants started to grow more quickly in B5 supplemented with 6-BA 0.51.0 mg/L and growth hormone(NAA or IBA),and some... |
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| Keywords: | biological engineering Prunus domestica L. tissue culture hormone |
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