| Construction of a Plasmid and a Standard Curve for Real-time Quantitative PCR of the Cold-induced Cor3 Gene from Volvariella volvacea |
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| 引用本文： | WANG Hong CHEN Mingjie. Construction of a Plasmid and a Standard Curve for Real-time Quantitative PCR of the Cold-induced Cor3 Gene from Volvariella volvacea[J]. 食用菌学报, 2007, 14(3): 20-23 |
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| 作者姓名： | WANG Hong CHEN Mingjie |
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| 作者单位： | Key Laboratory of Agricultural Genetics and Breeding of Shanghai; Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences. Shanghai 201106, China |
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| 基金项目： | Sponsored by the Shanghai Academy of Agricultural Science and Shanghai Science Committee （No. 003912076） |
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| 摘 要： |
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| 关 键 词： | 草菇 质粒 PCR Cor3基因 冷诱导 标准曲线 |
| 收稿时间： | 2007-05-18 |
| 修稿时间： | 2007-07-15 |
Construction of a Plasmid and a Standard Curve for Real-time Quantitative PCR of the Cold-induced Cor3 Gene from Volvariella volvacea |
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| Abstract: | A plasmid containing target DNA and a standard curve for real-time quantitative PCR of the coldinduced Cor3 gene of Volvariella volvacea were constructed. These will provide the basis for further research on Cor3 gene expression at low temperature, and ultimately for assigning a role for the gene in the low temperature autolysis of V. volvacea. |
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| Keywords: | Volvariella volvacea Cold induction Cor3 gene Quantitative PCR Plasmid Standard curve |
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