Phenotypic and genotypic characterization of Flavobacterium psychrophilum from Finnish fish farms |
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| Authors: | J Madetoja M-L Hänninen V Hirvelä–Koski I Dalsgaard & T Wiklund |
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| Institution: | Department of Biology, Institute of Parasitology, Åbo Akademi University, Åbo, Finland;Faculty of Veterinary Medicine, Department of Food and Environmental Hygiene, Helsinki University, Finland;Oulu Regional Laboratory, National Veterinary and Food Research Institute, Oulu, Finland;Fish Disease Laboratory, Danish Institute for Fisheries Research, Frederiksberg, Denmark |
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| Abstract: | Finnish isolates ( n =37) of Flavobacterium psychrophilum isolated from farmed salmonids were studied using phenotypic and genotypic characteristics. The characteristics of isolates were compared with the characteristics of Swedish and Estonian F. psychrophilum isolates and the type strain, F. psychrophilum NCIMB 1947T. Multiple isolates from eight disease outbreaks were examined to determine differences between isolates from a single outbreak. The F. psychrophilum isolates represented a biochemically homogeneous group. However, some minor differences in biochemical and physiological characteristics were observed. Seven different antigenic patterns among Finnish isolates were detected and the results suggest a new serotype of F. psychrophilum . Using Cla Ι, Hae ΙΙΙ and Pvu ΙΙ restriction enzymes in ribotyping analyses 13 different genotypes were demonstrated and a possible relationship between serotype Fd and genotype F1 was determined. There were no significant differences between the isolates from Finland, Estonia, Sweden and the type strain of F. psychrophilum . |
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| Keywords: | characterization Finland Flavobacterium psychrophilum rainbow trout fry syndrome (RTFS) ribotyping serotyping |
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