| 美人指葡萄不定芽离体诱导再生植株的研究 |
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| 引用本文： | 陶建敏,庄智敏,章镇,耿其芳,蔡斌华.美人指葡萄不定芽离体诱导再生植株的研究[J].果树学报,2005,22(5):551-554. |
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| 作者姓名： | 陶建敏 庄智敏 章镇 耿其芳 蔡斌华 |
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| 作者单位： | 南京农业大学园艺学院,南京,210095 |
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| 基金项目： | 江苏省科技厅、省农业资源开发局科技推广资助项目。 |
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| 摘 要： | 6-BA、TDZ与IBA不同浓度组合对美人指葡萄叶片、叶柄和茎段不定芽离体器官发生途径诱导再生植株有不同的影响。以茎段为外植体在MS+6-BA1.0mg/L+IBA0.01mg/L培养基上分化效果最好,再生率75.00%;叶片、叶柄分别在MS+6-BA3.0mg/L+IBA0.10mg/L和MS+6-BA2.0mg/L+IBA0.05mg/L培养基上分化效果较好。TDZ和IBA研究的几种组合都能诱导不定芽再生,但再生率不高。
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| 关 键 词： | 美人指葡萄 不定芽 植株再生 |
| 文章编号： | 1009-9980(2005)05-551-03 |
| 修稿时间： | 2005年1月11日 |
Study on plant regeneration from in vitro culture of Vitis vinifera cv.Manicure Finger |
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| Tao Jian-min,ZHANG Zhi-min,ZHANG Zhen,GENG Qi-fang,CAI Bin-hua.Study on plant regeneration from in vitro culture of Vitis vinifera cv.Manicure Finger[J].Journal of Fruit Science,2005,22(5):551-554. |
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| Authors: | Tao Jian-min ZHANG Zhi-min ZHANG Zhen GENG Qi-fang CAI Bin-hua |
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| Abstract: | Plant regeneration were induced from leaf, petiole and stem explants of grape cultivar Manicure finger (Vitis vinifera L.) with MS medium supplemented different combination of 6-BA, TDZ and IBA through organogenesis. The results indicated that the highest regenerating rate of adventitious buds were obtained when stem explants were incubated on MS media with 6-BA 1.0 mg/L+IBA 0.01 mg/L, it reached 75.00%. The optimum media for inducing adventitious buds from leaf and petiole explants were MS+6-BA 3.0 mg/L+IBA 0.1 mg/L and MS+6-BA 2.0 mg/L+IBA 0.05 mg/L. The initiation media containing various concentrations of TDZ and IBA could induce adventitious buds from leaf, petiole and stem explants, but the rates were not so high. |
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| Keywords: | Manicure Finger Grape Adventitious buds Plant regeneration |
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