不同品种育成猪基线免疫性状的比较

为了比较不同品种育成猪基线免疫指标的差异,研究选用巴马香猪、本地黑猪和杜长大3个品种育成猪,采用显微镜平板计数法计数红、白细胞总数;血涂片法测定白细胞分类计数;EA花环形成试验检测B淋巴细胞的百分率;流式细胞仪检测T淋巴细胞亚群百分率。结果表明:巴马香猪红细胞总数(RBC)、中性粒细胞百分率(GR%)、嗜酸性粒细胞百分率(EO%)以及T淋巴细胞亚群CD4+、CD8+百分率(CD4+%、CD8+%)等5项指标与本地黑猪、杜长大t检验差异显著(P<0.05),其中RBC和GR%显著高于本地黑猪和杜长大,EO%以及CD3+%、CD4+%、CD8+%显著低于本地黑猪和杜长大(P<0.05)。B淋巴细胞百分率(BLY%)显著低于本地黑猪,嗜碱性粒细胞(BASO%)显著低于杜长大,而白细胞总数(WBC)、淋巴细胞百分率(LY%)、单核细胞百分率(MO%)和CD4+/CD8+等4项指标与本地黑猪、杜长大基本一致。对本地黑猪与杜长大进行比较发现,WBC与BYL%2项差异显著。说明品种是影响基线免疫性状的一个重要因素,但对同一品种猪,各项指标间没有相关性。

Comparative Study of Baseline Immune Performance in Different Breed Growing Pigs

In order to compare the baseline immune indexes of different breeds pigs, We selected the Bama pig, the Local Black pig and the Duroc×Large White×Landrace pig as animal model, taking the Neubauer chamber method to count the total RBC and WBC, the microscopy of stained blood smear method to detect differential counts of the WBC, the Erythocyte antibody rosette test to detect the bone marrow dependent lymphocyte (B Lymphocyte) and the flow cytometry method to detect the thymus dependent lymphocyte subset. The results showed that the total RBC, percentage of neutrophils and eosinophils (GR%, EO%) as well as percentage of CD4+ and CD8+(CD4+%, CD8+%) in the Bama pig are different from the Local Black pig and the Duroc×Large White×Landrace pig (P < 0.05), both of the total RBC and GR% were higher than those of the Local Black pig and the Duroc×Large White×Landrace pig, on the contrary, all of the EO% as well as CD4+% and CD8+% were lower than of the Local Black pig and the Duroc×Large White×Landrace pig, only the percentage of the bone marrow dependent lymphocyte (BLY% ) was lower than the Local Black and the percentage of basophils (BASO%) was lower than those of the Duroc×Large White×Landrace pig; The other indexes such as the total WBC, percentage of lymphocytrs and monocytes (LY%, MO%) , CD4+/CD8+ were not different from the other two breeds. We compared the baseline immune index between the Local Black pig and the Duroc×Large White×Landrace pig, and found that only the total WBC and the BYL% are higher than the Duroc×Large White×Landrace pig. We concluded that the breed was an important factor that impacted the baseline immune characteristic in pigs,but the change of immune measures had no correlated to each other in individual pig.