| 同步检测为害百合种球3种检疫性病毒的多重RT-PCR方法 |
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| 引用本文： | 杨翠云,张丽莉,于翠,戚龙君.同步检测为害百合种球3种检疫性病毒的多重RT-PCR方法[J].植物病理学报,2009,39(3):321-324. |
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| 作者姓名： | 杨翠云 张丽莉 于翠 戚龙君 |
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| 作者单位： | 1 上海出入境检验检疫局, 上海 200135;2 西北农林科技大学, 杨凌 712100 |
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| 基金项目： | 上海市科委技术标准专项项目，国家质检总局科技项目 |
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| 摘 要： | The multiplex RT-PCR approach was developed for simultaneous detections of Arabis mosaic virus(ArMV),Strawberry latent ringspot virus(SLRSV)and Lily symptomless virus(LSV)from the imported lily bulbs.The results indicated that good specificity and sensitivity for simultaneous detection were obtained.The ultimate of RNA detection with three viruses mixture was 305 pg.The ultimate of RNA detection with ArMV,SLRSV and LSV were 156.0,13.4 pg and 1.12 ng respectively.This approach has potential to be used in quarantine of imports and exports.
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| 收稿时间： | 2008-10-10 |
Simultaneous detection of three quarantine viruses infecting lily bulbs by multiplex RT-PCR |
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| YANG Cui-yun,ZHANG Li-li,YU Cui,QI Long-jun.Simultaneous detection of three quarantine viruses infecting lily bulbs by multiplex RT-PCR[J].Acta Phytopathologica Sinica,2009,39(3):321-324. |
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| Authors: | YANG Cui-yun ZHANG Li-li YU Cui QI Long-jun |
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| Institution: | 1 Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai 200135, China;2 Northwest Agriculture and Forest University, Yangling 712100, China |
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| Abstract: | The multiplex RT-PCR approach was developed for simultaneous detections of Arabis mosaic virus(ArMV),Strawberry latent ringspot virus(SLRSV)and Lily symptomless virus(LSV)from the imported lily bulbs.The results indicated that good specificity and sensitivity for simultaneous detection were obtained.The ultimate of RNA detection with three viruses mixture was 305 pg.The ultimate of RNA detection with ArMV,SLRSV and LSV were 156.0,13.4 pg and 1.12 ng respectively.This approach has potential to be used in quarantine of imports and exports. |
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| Keywords: | Arabis mosaic virus Strawberry latent ringspot virus Lily symptomless virus multiplex RT-PCR simultaneous detection |
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