| IGF-1和IGF-1R基因在辽宁绒山羊皮肤组织中的表达 |
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| 引用本文： | 刘海英,宋先忱,姚玉园,丛玉艳,韩迪,李丰田,袁缨. IGF-1和IGF-1R基因在辽宁绒山羊皮肤组织中的表达[J]. 黑龙江畜牧兽医, 2012, 0(11): 7-9,179 |
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| 作者姓名： | 刘海英 宋先忱 姚玉园 丛玉艳 韩迪 李丰田 袁缨 |
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| 作者单位： | 沈阳农业大学畜牧兽医学院;辽宁省畜牧科学研究院;大连顺祥牧业有限公司 |
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| 基金项目： | 国家自然科学基金项目(31101734);“十一五”国家科技支撑计划项目(2009BADA5B02);辽宁省博士启动基金项目(20101097);沈阳农业大学校国家自然科学基金启动基金项目(20102004) |
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| 摘 要： | 为了研究类胰岛素生长因子1(IGF-1)和类胰岛素生长因子1受体(IGF-1R)基因在绒山羊绒毛生长不同阶段皮肤组织中的表达差异,试验采用实时荧光定量PCR技术对非产绒期和产绒期辽宁绒山羊皮肤中IGF-1和IGF-1R基因的表达进行了测定。结果表明:辽宁绒山羊皮肤中IGF-1和IGF-1R mRNA的表达量在产绒期均极显著高于非产绒期(P<0.01),产绒期IGF-1、IGF-1RmRNA的表达水平分别是非产绒期的8.30倍和7.49倍。说明IGF-1和IGF-1R基因可能与山羊绒周期性生长有关。
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| 关 键 词： | 辽宁绒山羊 皮肤 类胰岛素生长因子1(IGF-1) 类胰岛素生长因子1受体(IGF-1R) 实时荧光定量PCR |
The expressions of IGF-1 and IGF-1R gene in skin tissues of Liaoning cashmere goats |
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| LIU Hai-ying,SONG Xian-chen,YAO Yu-yuan,CONG Yu-yan,HAN Di,LI Feng-tian,YUAN Ying. The expressions of IGF-1 and IGF-1R gene in skin tissues of Liaoning cashmere goats[J]. Heilongjiang Animal Science And veterinary Medicine, 2012, 0(11): 7-9,179 |
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| Authors: | LIU Hai-ying SONG Xian-chen YAO Yu-yuan CONG Yu-yan HAN Di LI Feng-tian YUAN Ying |
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| Affiliation: | 1(1.College of Animal Science and Veterinary Science,Shenyang Agricultural University,Shenyang 110866, China;2.Institute of Animal Husbandry of Liaoning Province,Liaoyang 111000,China;3.Dalian Shunxiang Animal Husbandry Co.,Ltd.,Dalian 116301,China) |
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| Abstract: | To study the expression differences of IGF-1 and IGF-1R gene in skin of cashmere goats at different growing periods,the expression of IGF-1 and IGF-1R gene in skin tissue of Liaoning cashmere goats at the cashmere non-growing period and the cashmere growing period were measured by using Real-time fluorescence quantitative PCR.The results showed that the expression levels of IGF-1 and IGF-1R mRNA were significantly higher at the cashmere growing period than that of at the cashmere non-growing period(P<0.01).The expression levels of IGF-1 and IGF-1R mRNA in skin tissue at the cashmere growing period were 8.30 and 7.49 times of the cashmere no-growing period,respectively.The results indicate that the IGF-1 and IGF-1R gene may be related to the cyclical growth of cashmere. |
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| Keywords: | Liaoning cashmere goat skin IGF-1 IGF-1R Real-time fluorescence quantitative PCR |
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