油菜花粉谷蛋白酶解物的制备

以陈放1年的油菜蜂花粉为原料,对破壁脱脂后的花粉中各组分蛋白含量进行了研究,其中谷蛋白的含量约占总蛋白含量的55.7%,清蛋白为39.O%,球蛋白和醇蛋白只有3.2%和2.1%.以谷蛋白为原料,在单因素试验的基础上,利用响应面分析法对油菜花粉谷蛋白最佳酶解条件进行了研究.结果表明,Alcalase碱性蛋白酶水解油菜花粉谷蛋白的最佳水解条件为底物浓度6%,pH值9.0,水解温度60℃,酶底比1460U/g,水解时间2h,谷蛋白酶法改性后抗羟基自由基的活性能达到70.0%.

Preparation of peptides hydrolyzed from rape pollen glutelin

Protein fractionations of cell-fragmentated and defatted rape pollen stored for more than 12 months were investigated. Rape pollen glutelin was hydrolyzed with alcalase and the crude peptides of rape pollen were obtained. The scavenging effects of rape pollen glutelin and its hydrolysate on hydroxyl free radicals (·OH) in the deoxyribose-iron system was studied. Results show that glutelins and albumins are found to be the predominant proteins in rape pollen, accounting for 55.7% and 39.0% of total proteins, while globulins and prolamins are 3.2% and 2.1%. respectively. The optimum conditions of alcalase enzymatic hydrolysis were determined by single-factor analysis and response surface methodology as follows: pH9, hydrolyzing temperature 50℃, enzyme concentration 1460 U per gram of substrate, concentration of substrate 6%, hydrolyzing time 2 h. In the antioxidation test, the inhibition rate·OH of glutelin was 25.1%. After being hydrolyzed, the inhibition rate of·OH of glutelin oligopeptide was remarkably increased to reach 70.0%.