日本红枫组织培养技术研究

对日本红枫茎段组织培养过程中的外植体灭菌、启动培养、增殖培养和生根培养等进行了研究。结果表明:日本红枫外植体经1.0%次氯酸钠灭菌4 min后,成活率达到最高,为78.9%;以MS为基本培养基筛选出适宜日本红枫的启动培养基为MS+6-BA 1.0 mg·L~(-1)+NAA 0.1 mg·L~(-1);在MS培养基中附加0.005 mg·L~(-1)TDZ时,可获得最佳的增殖效果,最佳的生根培养基为1/2MS+IBA 0.2 mg·L~(-1)+蔗糖30 g·L~(-1)+琼脂5g·L~(-1),生根率可达97.8%。幼苗经炼苗后移栽,成活率在90%以上。

Tissue Culture of Acer palmatum atropurpureum

The stem segments of Acer palmatum atropurpureum was used as explants to study sterilization techniques,initial culture,proliferation and rooting in vitro.The results showed that the highest survival rate of the explants was 78.9%after the explants sterilized in 1.0%NaClO_3 for 4 min;The optimum initiation media was MS medium supplemented with 1.0 mg·L~（-1） 6-BA and 0.1 mg·L~（-1） NAA;The MS medium containing 0.005 mg·L~（-1） TDZ was the most efficient in the proliferation of Acer palmatum atropurpureum;The ratio of rhizogenesis was 97.8%in the optimum rooting medium of 1/2 MS medium supplemented with 0.2 mg·L~（-1） IBA,30 g·L~（-1） sugar and 5 g·L~（-1） agar,the survival ratio of transplanting was 90%after training seedling.