| 水杨酸诱导云烟85基因表达差减文库的构建及初步分析* |
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| 引用本文： | 董霞,李文正,黄夸克,蓝建强. 水杨酸诱导云烟85基因表达差减文库的构建及初步分析*[J]. 云南农业大学学报(自然科学版), 2005, 20(6): 771-773 |
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| 作者姓名： | 董霞 李文正 黄夸克 蓝建强 |
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| 作者单位： | 云南农业大学东方蜜蜂研究所，云南省烟草科学研究所，云南农业大学东方蜜蜂研究所，云南农业大学研究生处 云南昆明650201，四川大学生命科学学院，四川成都610064，云南玉溪653100，云南昆明650201，云南昆明650201 |
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| 基金项目： | 云南省烟草公司资助项目(03A010515) |
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| 摘 要： | 利用水杨酸（SA）诱导烟草抗病相关基因表达,以此为目标样本,以清水喷施为对照样本,进行抑制差减杂交,构建烟草抗病基因表达文库。结果显示,插入片段主要集中在150～500bp之间。随机挑选12个克隆进行测序,结果有7条与已知的系统获得性抗病基因同源,1条与病程相关蛋白PR1a同源,1条与光系统II的促氧蛋白一个亚基有较高同源性,1条与水通道蛋白基因（aquaporin 1）同源, 1条与Ert13基因有关,1条未找到同源序列,是新的cDNA片段。
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| 关 键 词： | 水杨酸 SSH cDNA文库 烟草 |
| 文章编号： | 1004-390X（2005）06-0771-03 |
| 收稿时间： | 2005-09-12 |
| 修稿时间： | 2005-09-12 |
Construction of cDNA Library and Analyses from the SA-stimulated Tobacco |
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| DONG Xia, LI Wen-zheng, HUANG Kua-ke , LANG Jiang-qiang. Construction of cDNA Library and Analyses from the SA-stimulated Tobacco[J]. Journal of Yunnan Agricultural University, 2005, 20(6): 771-773 |
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| Authors: | DONG Xia LI Wen-zheng HUANG Kua-ke LANG Jiang-qiang |
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| Affiliation: | 1. Eastern Bee Research Institute, Y A U, Kunming 650201, China; 2. Yunnan Tobacco Science Research Institute, Yuxi 653100, China; 3. College of Life Science, Sichuan University, Chengdu 610064, China; 4. Department Education on Postgraduate,Y A U, Kunming 650201, China |
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| Abstract: | The SSH library of differently expressed cDNA was constructed, in which the tobacco with salicylic acid stimulated as tester, and one with water sprayed as driver. Analysis showed that most of the inserted fragments were 150 - 500 bp. Twelve clones were selected randomly and sequenced. The BLASTN homology analysis on GenBank revealed that seven clones have homology with tobacco resistance genes, one clone is homologous with PR1 a, other three are homologous with oxygen-evolving protein, aquaporin 1 and Ertl3 gene, only one is a new eDNA with no homologous sequence. |
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| Keywords: | SA SSH cDNA library tobacco |
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