| 观赏植物"孩儿莲"及其近缘植物的RAPD分析 |
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| 引用本文： | 苏媚,方向明,王雪芹,阚海玲,刘文倩,谈建中.观赏植物"孩儿莲"及其近缘植物的RAPD分析[J].安徽农业科学,2010,38(10):5006-5008. |
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| 作者姓名： | 苏媚 方向明 王雪芹 阚海玲 刘文倩 谈建中 |
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| 作者单位： | 苏州大学建筑与城市环境学院园艺系,江苏,苏州,215123;苏州大学建筑与城市环境学院园艺系,江苏,苏州,215123;苏州大学建筑与城市环境学院园艺系,江苏,苏州,215123;苏州大学建筑与城市环境学院园艺系,江苏,苏州,215123;苏州大学建筑与城市环境学院园艺系,江苏,苏州,215123;苏州大学建筑与城市环境学院园艺系,江苏,苏州,215123 |
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| 基金项目： | 苏州市农业科技攻关项目 |
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| 摘 要： | 目的]了解八角属植物披针叶八角种内的遗传多样性和亲缘关系。方法]以＂孩儿莲＂等13个植物类型为试验材料,以木莲、白玉兰、香樟为外类群对照进行RAPD分析。结果]从35条随机引物中,筛选出多态性强、带型清晰、重复性好的随机引物20条,13个供试植物类型共扩增出154条DNA谱带,其中共有条带47条。利用NTSYSpc2.10e软件进行数据处理和分析,相似系数的变异在66.2%～90.3%。用UPGMA法进行聚类分析,可将13个供试植物类型划分为3个类群。结论]＂孩儿莲＂等13个供试植物有较近的亲缘关系,其遗传差异与地理因素之间未见确定联系。
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| 关 键 词： | 八角科植物 孩儿莲 RAPD 亲缘关系 |
RAPD Analysis of Ornamental Plant Haierlian and Its Closely-related Plants |
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| SU Mei et al.RAPD Analysis of Ornamental Plant Haierlian and Its Closely-related Plants[J].Journal of Anhui Agricultural Sciences,2010,38(10):5006-5008. |
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| Authors: | SU Mei |
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| Institution: | SU Mei et al (Department of Horticulture,School of Architecture , Urban Environment,Soochow University,Suzhou,Jiangsu 215123) |
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| Abstract: | Objective] The research aimed to study genetic diversity and relationship in Illicium laceolatum of Illicium L..Method] 13 plant types of Haierlian were used as test materials to make RAPD analysis by using Manglietia fordiana,Magnolia denudate and Cinnamomum camphora as outgroups.Result] 20 primers with high polymorphism,clear bands and good repeatability were selected from 35 random primers.154 DNA bands were amplified from 13 species of test plants by using selected 20 primers including 47 common band... |
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| Keywords: | RAPD Illiciaceae plant Haierlian RAPD Genetic relationship |
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