Dinitrogen fixation activity of Azorhizobium caulinodans in the rice (Oryza sativa L.) rhizosphere assessed by nitrogen balance and nitrogen-15 dilution methods |
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Authors: | C Van Nieuwenhove R Merckx Lieven Van Holm Karel Vlassak |
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Institution: | (1) Faculty of Agricultural and Applied Biological Sciences, Laboratory of Soil Biology and Soil Fertility, Kard. Mercierlaan 92, 3001 Heverlee, Belgium Fax: +32-16-321997, BE;(2) Faculty of Agricultural and Applied Biological Sciences, Laboratory of Soil Biology and Soil Fertility, K.U. Leuven, Belgium, BE;(3) Institute of Fundamental Studies, Kandy, Sri Lanka, LK |
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Abstract: | Azorhizobium caulinodans was directly inoculated onto rice plants in three short-term pot trials. Addition of increasing amounts of sucrose (23, 46,
92 kg ha–1) did not influence the N economy of the A. caulinodans-rice association during the early vegetative growth stage. A. caulinodans inoculation alone and in combination with the highest amount of sucrose had a significantly positive effect on the N balance,
with small but significant N gains in the system. Application of 60 kg urea-N ha–1 had a negative impact on the N economy of the inoculated treatments. N losses increased and the amount of atmospheric N2 fixed and incorporated decreased significantly as compared to the amounts under the 20 kg urea-N ha–1 regime. However, N losses were low – a maximum of 8% – at the early vegetative growth stage under the conditions of the experiments.
C limitation does not seem to be a limiting factor for the incorporation of fixed N2 in this bacteria-plant association. Biological N2 fixation caused by A. caulinodans inoculation was responsible for 14% of the plant N at the vegetative growth stage and under low N conditions.
Received: 30 January 2000 |
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Keywords: | Azorhizobium caulinodans Rice Seedling inoculation Nitrogen derived from the atmosphere Total nitrogen difference method |
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