| 应用21个微卫星标记监测马身猪遗传多样性变化趋势 |
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| 引用本文： | 曹果清,李步高,石建中,刘宏,薛尚军,周忠孝.应用21个微卫星标记监测马身猪遗传多样性变化趋势[J].畜牧兽医学报,2010,41(8). |
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| 作者姓名： | 曹果清 李步高 石建中 刘宏 薛尚军 周忠孝 |
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| 作者单位： | 1. 山西农业大学动物科技学院,太谷,030801
2. 大同市种猪场,大同,037000 |
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| 基金项目： | 山西省攻关项目，山西省归国留学人员资助项目 |
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| 摘 要： | 本研究旨在通过对不同年份马身猪群体遗传多样性变化趋势分析,评价马身猪保种效果,制定有效的保种措施。选用FAO-ISAG联合推荐的21个微卫星标记分析不同年份马身猪群体的遗传多样性。结果表明,在马身猪群体中,共检测到105个等位基因,平均等位基因数为5个,平均有效等位基因数为2.244 0,平均观察杂合度和PIC分别为0.317 6和0.391 9。从1999年到2007年,平均等位基因数从4.285 7下降到3.428 6,平均有效等位基因数从2.509 5下降到1.977 0,平均观察杂合度从0.311 6下降到0.298 1,平均多态信息含量从0.441下降到0.341。马身猪群体基因纯度较高,遗传多样性有逐年降低趋势,在今后的保种和利用工作中,应对马身猪群体的遗传多样性进行实时监测,在世代更替中注意低频率等位基因的保护。
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| 关 键 词： | 马身猪 遗传多样性 微卫星标记 遗传监测 |
Genetic Monitoring on Genetic Diversity of Mashen Pig Using 21 Microsatellite Markers |
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| CAO Guo-qing,LI Bu-gao,SHI Jian-zhong,LIU Hong,XUE Shang-jun,ZHOU Zhong-xiao.Genetic Monitoring on Genetic Diversity of Mashen Pig Using 21 Microsatellite Markers[J].Acta Veterinaria et Zootechnica Sinica,2010,41(8). |
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| Authors: | CAO Guo-qing LI Bu-gao SHI Jian-zhong LIU Hong XUE Shang-jun ZHOU Zhong-xiao |
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| Institution: | CAO Guo-qing1,LI Bu-gao 1,SHI Jian-zhong2,LIU Hong2,XUE Shang-jun2,ZHOU Zhong-xiao1(1.College of Animal Science and Veterinary Medicine,Shanxi Agricultural University,Taigu 030801,China,2.Datong Pig Breeding Farm,Datong 037000,China) |
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| Abstract: | The aim of this study was to evaluate the conserved effect for Mashen pig by analyzing the trend of genetic diversity of Mashen pig populations during 1999 to 2007 and to make the effective measures for conservation.A total of 21 microsatellite markers recommended by FAO-ISAG were used to study the genetic diversity and the changing trend of Mashen pig.The results showed that there were 105 alleles detected in the overall Mashen pig population.The mean allele number per locus and mean effective allele numbe... |
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| Keywords: | Mashen pig genetic diversity microsatellite marker genetic monitoring |
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