| B96-II对3种枯萎病的防治效果及拮抗物质初步分析 |
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| 引用本文： | 马利平,乔雄梧,高芬,郝变青. B96-II对3种枯萎病的防治效果及拮抗物质初步分析[J]. 华北农学报, 2006, 0(4) |
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| 作者姓名： | 马利平 乔雄梧 高芬 郝变青 |
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| 作者单位： | 山西省农业科学院山西省农药重点实验室 山西太原030031 |
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| 基金项目： | 国家科技攻关计划项目(2001BA539C)，山西省自然科学基金项目(20051076)，山西省科技攻关项目(2006031033) |
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| 摘 要： | 枯萎病拮抗菌B96-II(Bacillussp)自沤肥浸渍液中分离筛选获得。进一步试验证明,该菌对黄瓜枯萎病菌、西瓜枯萎病菌、青椒枯萎病菌等有明显抑制效果。可观察到的抑菌现象有:对孢子的溶解作用;对孢子壁的破坏作用;对芽管的生长抑制和对菌丝致畸等作用,对上述3种病菌孢子萌发抑制率为56.2%,57.3%,54.7%,对芽管的抑制率分别为88.5%,87.5%,79.5%。拮抗物质之一是几丁质酶。
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| 关 键 词： | 枯萎病 尖孢镰刀菌 拮抗菌 抑制作用 几丁质酶 |
Inhibition Effects of Antagonist Stain B96-II to Different Fusarium Wilt and Its Main Inhibiting Substance |
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| MA Li-ping,QIAO Xiong-wu,GAO Fen,HAO Bian-qing. Inhibition Effects of Antagonist Stain B96-II to Different Fusarium Wilt and Its Main Inhibiting Substance[J]. Acta Agriculturae Boreali-Sinica, 2006, 0(4) |
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| Authors: | MA Li-ping QIAO Xiong-wu GAO Fen HAO Bian-qing |
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| Abstract: | A antagonistic strain of Bacillus sp(B96-II) against Fusarium wilt was isolated from compost extract.Further tests proved that the B96-II could effectively inhibit the development of 3 Fusarium wilts in cucumber,water melon,paprika,etc.The phenomena of conidia lysogenesis,conidia wall destruction,inhibition,and deformation of hyphal growth were observed.The inhibition rates of conidia germination of the above mentioned pathogens were 56.2%,57.3%,and 54.7%,and the inhibition rates of germination tube growth were 88.5%,87.5%,and 79.5%.Chitinase was detected as one of the main inhibiting substances. |
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| Keywords: | Soil born disease Fusarium oxysporum Antagonist Chitinase Lysogenesis |
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