| 龟甲竹染色体C-分带、荧光原位杂交及其核型分析 |
| |
| 引用本文： | 徐川梅,郑华威,王骢,汤定钦. 龟甲竹染色体C-分带、荧光原位杂交及其核型分析[J]. 林业科学研究, 2009, 22(5): 691-695 |
| |
| 作者姓名： | 徐川梅 郑华威 王骢 汤定钦 |
| |
| 作者单位： | 江省现代森林培育技术重点实验室,浙江林学院,浙江,临安,311300;江省现代森林培育技术重点实验室,浙江林学院,浙江,临安,311300;江省现代森林培育技术重点实验室,浙江林学院,浙江,临安,311300;江省现代森林培育技术重点实验室,浙江林学院,浙江,临安,311300 |
| |
| 基金项目： | 国家自然基金,浙江林学院启动基金,浙江林学院森林培育重中之重学科开放基金(20016)项目 |
| |
| 摘 要： | 利用Giemsa C-分带和荧光原位杂交的方法对龟甲竹根尖细胞有丝分裂中期染色体进行了研究.结果表明:龟甲竹的核型公式为2n=48=26m+14sm+4st(2SAT)+4t,核型分类属ⅡB型;带型公式为2n=48=4CI+2CI+T+2CT++IOCT++4I+2I++6T++6C+8T+2IT+2I+T,每条染色体都显示出了可以明显区别的特征带,带纹强弱差异明显.以45S rDNA为探针对龟甲竹根尖染色体进行了荧光原位杂交,杂交位点定位于1对染色体上.将染色体c-带带型和45S rDNA FISH带型相结合可以将龟甲竹的每条染色体区分开.
|
| 关 键 词： | 龟甲竹 染色体 C-分带 荧光原位杂交 |
| 收稿时间： | 2008-12-03 |
G iem sa C-band ing, F ISH and Karyotype Ana lysis ofPhyllosta chys pubescens var. heterocycla Chrom osom e |
| |
| XU Chuan-mei,ZHENG Hua-wei,WANG Cong and TANG Ding-qin. G iem sa C-band ing, F ISH and Karyotype Ana lysis ofPhyllosta chys pubescens var. heterocycla Chrom osom e[J]. Forest Research, 2009, 22(5): 691-695 |
| |
| Authors: | XU Chuan-mei ZHENG Hua-wei WANG Cong TANG Ding-qin |
| |
| Affiliation: | Key Lab forModern Silvicultural Techno logy of Zh ejiang Province, Zhejiang ForestryUn ivers ity, Lin. An 311300, Zh ejiang, Ch ina;Key Lab forModern Silvicultural Techno logy of Zh ejiang Province, Zhejiang ForestryUn ivers ity, Lin. An 311300, Zh ejiang, Ch ina;Key Lab forModern Silvicultural Techno logy of Zh ejiang Province, Zhejiang ForestryUn ivers ity, Lin. An 311300, Zh ejiang, Ch ina;Key Lab forModern Silvicultural Techno logy of Zh ejiang Province, Zhejiang ForestryUn ivers ity, Lin. An 311300, Zh ejiang, Ch ina |
| |
| Abstract: | Giemsa C-band ing and FISH analysis of the Ph. pubescens var. heterocycla chromosomes from root tipswere carried ou t in this research. The resu lts showed that the C-band ing karyotypewou ld be 2n= 48= 4CI+ 2CI+ T+ 2CT+ + 10CT+ + 4I+ 2I+ + 6T+ + 6C+ 8T + 2IT + 2I+ T. The specific C-band ing points on each chromosomecou ld be distinguished and the d ifferences ofC-band ing intensity a lso could be recognized. 45S rDNA was used asprobe for FISH analysis, and one couple of hybridization signals was d isp layed on the chromosome centrimere. Inthis research we have a conclusion that chromosomes ofPh. pubescens var. heterocycla can be easily distinguishedand identified by Giemsa C-band ing and FISH approaches. |
| |
| Keywords: | Phyllosta chys pubescens var. heterocycla chromosome C-band ing fluorescent in situ hybridization( FISH) |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《林业科学研究》浏览原始摘要信息 |
|
点击此处可从《林业科学研究》下载免费的PDF全文 |
|
